Catalog No: OKCD01092 (Formerly GWB-KBBKR8)
Size:96WELLS
Price: $750.00
SKU
OKCD01092
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Datasheets/ManualsPrintable datasheet for EML2 ELISA Kit (Human) (OKCD01092)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Echinoderm Microtubule Associated Protein Like Protein 2 (EML2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Echinoderm Microtubule Associated Protein Like Protein 2 (EML2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Echinoderm Microtubule Associated Protein Like Protein 2 (EML2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Echinoderm Microtubule Associated Protein Like Protein 2 (EML2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Echinoderm Microtubule Associated Protein Like Protein 2 (EML2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Echinoderm Microtubule Associated Protein Like Protein 2 (EML2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-EML2 Microplate96 Wells (12 x 8 Well Strips)
EML2 Lyophilized Standard2 x 20 ng
100X Biotinylated EML2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.113 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Echinoderm Microtubule Associated Protein Like Protein 2 (EML2). No significant cross-reactivity or interference between Echinoderm Microtubule Associated Protein Like Protein 2 (EML2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolEML2
Gene Full NameEMAP like 2
Alias Symbolsechinoderm microtubule associated protein like 2;echinoderm microtubule-associated protein-like 2;echinoderm MT-associated protein (EMAP)-like protein 70;ELP70;EMAP2;EMAP-2;microtubule-associated protein like echinoderm EMAP.
NCBI Gene Id24139
Protein NameEchinoderm microtubule-associated protein-like 2
Description of TargetTubulin binding protein that inhibits microtubule nucleation and growth, resulting in shorter microtubules.
Uniprot IDO95834
Protein Accession #NP_001180197
Nucleotide Accession #NM_001193268.1
Protein Size (# AA)649
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