EIF4EBP1 Antibody (OAAF01015)

Catalog No: OAAF01015 (Formerly GWB-ASC419)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using 4E-BP1 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of A549 cells, using 4E-BP1 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human 4E-BP1.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: 4E-BP1 antibody detects endogenous levels of total 4E-BP1 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:1000
• Reference: M. Gorre, Blood (ASH Annual Meeting Abstracts), Nov 2005; 106: 2772.
  Robert J. J. Biol. Chem., Jul 2007; 282: 21176 - 21186.
  Xiaoyan Zhou, Clin. Cancer Res., Oct 2004; 10: 6779 - 6788.

Target Info

• Gene Symbol: EIF4EBP1
• Gene Full Name: eukaryotic translation initiation factor 4E binding protein 1
• Alias Symbols: 4EBP1;4E-BP1;BP-1;eIF4E-binding protein 1;eukaryotic translation initiation factor 4E-binding protein 1;PHAS-I;phosphorylated heat- and acid-stable protein regulated by insulin 1.
• NCBI Gene Id: 1978
• Protein Name: Eukaryotic translation initiation factor 4E-binding protein 1
• Description of Target: Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
• Uniprot ID: Q13541
• Protein Accession #: NP_004086.1
• Molecular Weight: 12 kDa