EIF3H Antibody - N-terminal region (OAAB09189)

Catalog No: OAAB09189

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Western blot analysis of EIF3H (arrow) using rabbit polyclonal EIF3H Antibody (N-term) (Cat. #OAAB09189). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF3H gene.

EIF3H Antibody (N-term) (Cat. #OAAB09189)immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF3H Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Zebrafish
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: EIF3H Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation
  Cellular Location: Cytoplasm (By similarity).
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This EIF3H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-99 amino acids from the N-terminal region of human EIF3H.
• Predicted Homology Based on Immunogen Sequence: Cow; Human; Mouse; Rat; Zebrafish
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50

Target Info

• Gene Symbol: EIF3H
• Alias Symbols: EIF3S3, eIF3-p40, eIF3-gamma
• NCBI Gene Id: 8667
• Protein Name: Eukaryotic translation initiation factor 3 subunit H
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: O15372
• Protein Accession #: NP_003747.1
• Molecular Weight: 39930 Da