EAR3 ELISA Kit (Mouse) (OKCD01136)

Catalog No: OKCD01136 (Formerly GWB-KBBUD7)

Size:96WELLS

Mouse Ear3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	79-98%	80-89%	94-103%	87-98%
  EDTA plasma(n=5)	97-105%	88-96%	94-101%	80-92%
  heparin plasma(n=5)	85-95%	90-98%	83-98%	78-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A3 (RNASE3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease A3 (RNASE3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A3 (RNASE3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ribonuclease A3 (RNASE3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 62.5-4,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	80-97	81
  EDTA plasma(n=5)	78-103	96
  heparin plasma(n=5)	90-101	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribonuclease A3 (RNASE3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribonuclease A3 (RNASE3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Ear3 Microplate: 96 Wells (12 x 8 Well Strips)
  • Ear3 Lyophilized Standard: 2 x 4 ng
  • 100X Biotinylated Ear3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 28.1 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ribonuclease A3 (RNASE3). No significant cross-reactivity or interference between Ribonuclease A3 (RNASE3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Ear3
• Gene Full Name: eosinophil-associated, ribonuclease A family, member 3
• Alias Symbols: eosinophil cationic-type ribonuclease 3;eosinophil-associated ribonuclease 3;mR;mR3;MR-3;murine ribonuclease 3;ribonuclease, RNase A family 3;Rn;Rnase3.
• NCBI Gene Id: 53876
• Protein Name: Eosinophil cationic-type ribonuclease 3
• Description of Target: Eosinophil cationic-type ribonuclease 3
• Uniprot ID: O35290
• Protein Accession #: NP_059084
• Nucleotide Accession #: NM_017388.1
• Protein Size (# AA): 156