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Datasheets/Manuals | Printable datasheet for DIO2 Antibody (OAEB00859) |
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Predicted Species Reactivity | Human|Mouse|Porcine|Rat |
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Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | IHC |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | DIO2 |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Goat: 91%; Human: 100%; Rabbit: 91%; Rat: 91%; Sheep: 91% |
Peptide Sequence | EVKKHQNQEDRC |
Concentration | 0.5 mg/ml |
Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Application Info | Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Preliminary experiments gave an approx 65-70kDa band in Human Heart and Thyroid lysates after 0.05ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.5kDa according to NP_NP_000784.2 and of 34.6kDa according to NP_001007024.1. The 65-70kDa band was successfully blocked by incubation with the immunizing peptide. |
Reference | * Gumieniak O, Perlstein TS, Williams JS, Hopkins PN, Brown NJ, Raby BA, Williams GH.; Ala92 Type 2 Deiodinase Allele Increases Risk for the Development of Hypertension.; Hypertension. 2007 Jan 15; [Epub ahead of print]; Pubmed 17224473 |
Gene Symbol | Dio2|DIO2 |
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Gene Full Name | deiodinase, iodothyronine, type II|iodothyronine deiodinase 2 |
Alias Symbols | 5DII;AI324267;D2;deiodinase, iodothyronine type II;deiodinase, iodothyronine, type 2;deiodinase, iodothyronine, type II;deiodinase-2;deiodonase-2;DIOII;iodothyronine type II deiodinase;selenoprotein Y;SELENOY;SelY;thyroxine deiodinase, type II;TXDI2;type 2 DI;type 2 iodothyronine deiodinase;type II iodothyronine deiodinase;type-II 5' deiodinase;type-II 5'deiodinase;type-II 5'-deiodinase. |
NCBI Gene Id | 13371|1734|65162 |
Protein Name | Type II iodothyronine deiodinase |
Uniprot ID | Q92813 |
Protein Accession # | NP_000784.2 |
Molecular Weight | 30.5; 34.6 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "DIO2 Antibody (OAEB00859)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Porcine|Rat".
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How long will it take to receive "DIO2 Antibody (OAEB00859)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "DIO2 Antibody (OAEB00859)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "DIO2 Antibody (OAEB00859)"?
This target may also be called "5DII;AI324267;D2;deiodinase, iodothyronine type II;deiodinase, iodothyronine, type 2;deiodinase, iodothyronine, type II;deiodinase-2;deiodonase-2;DIOII;iodothyronine type II deiodinase;selenoprotein Y;SELENOY;SelY;thyroxine deiodinase, type II;TXDI2;type 2 DI;type 2 iodothyronine deiodinase;type II iodothyronine deiodinase;type-II 5' deiodinase;type-II 5'deiodinase;type-II 5'-deiodinase." in publications.
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What is the shipping cost for "DIO2 Antibody (OAEB00859)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "DIO2 Antibody (OAEB00859)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "DIO2 Antibody (OAEB00859)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "30.5; 34.6".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "DIO2 Antibody (OAEB00859)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Dio2|DIO2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Dio2|DIO2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Dio2|DIO2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Dio2|DIO2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Dio2|DIO2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Dio2|DIO2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.