DDB1 Antibody (OAEB01572)

Catalog No: OAEB01572 (Formerly GWB-5583AA)

Size:100UG

OAEB01572 (1 ug/ml) staining of NSO lysate (1E5 cells per lane). Detected by western blot using chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: DDB1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: C-DLIKVVEELTRIH
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:32000.
  Western Blot: Approx 130kDa band seen in HeLa, Jurkat and NSO (mouse) cell lysates. Recommended for use at 0.5-1.5 ug/ml.
• Reference: * Leung-Pineda V, Huh J, Piwnica-Worms H.; DDB1 targets Chk1 to the Cul4 E3 ligase complex in normal cycling cells and in cells experiencing replication stress; Cancer Res. 2009 Mar 15;69(6):2630-7.; Pubmed 19276361
  * Alekseev S, Luijsterburg MS, Pines A, Geverts B, Mari PO, Giglia-Mari G, Lans H, Houtsmuller AB, Mullenders LH, Hoeijmakers JH, Vermeulen W.; Cellular concentrations of DDB2 regulate dynamic binding of DDB1 at UV-induced DNA damage.; Mol Cell Biol. 2008 Dec;28(24):7402-13.; Pubmed 18936169
  * Luijsterburg MS, Goedhart J, Moser J, Kool H, Geverts B, Houtsmuller AB, Mullenders LH, Vermeulen W, van Driel R.; Dynamic in vivo interaction of DDB2 E3 ubiquitin ligase with UV-damaged DNA is independent of damage-recognition protein XPC.; J Cell Sci. 2007 Aug 1;120(Pt 15):2706-16.; Pubmed 17635991
  * Westbrook TF, Martin ES, Schlabach MR, Leng Y, Liang AC, Feng B, Zhao JJ, Roberts TM, Mandel G, Hannon GJ, Depinho RA, Chin L, Elledge SJ.; A genetic screen for candidate tumor suppressors identifies REST.; Cell. 2005 Jun 17;121(6):837-48.; Pubmed 15960972
  * Dualan R, Brody T, Keeney S, Nichols AF, Admon A, Linn S.; Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein.; Genomics 1995 Sep 1;29(1):62-9; Pubmed 8530102

Target Info

• Gene Symbol: Ddb1|DDB1
• Gene Full Name: damage specific DNA binding protein 1|damage-specific DNA binding protein 1
• Alias Symbols: damage-specific DNA binding protein 1, 127kDa;DDB p127 subunit;DDBA;DNA damage-binding protein 1;DNA damage-binding protein a;HBV X-associated protein 1;UV-damaged DNA-binding factor;UV-damaged DNA-binding protein 1;UV-DDB 1;UV-DDB1;WHIKERS;XAP1;XAP-1;xeroderma pigmentosum group E-complementing protein;XPCE;XPE;XPE-BF;XPE-binding factor.
• NCBI Gene Id: 1642|64470
• Protein Name: DNA damage-binding protein 1
• Uniprot ID: Q16531
• Protein Accession #: NP_001914.3
• Molecular Weight: 127