Cystatin B ELISA Kit (Human) (OKBB00539)

Catalog No: OKBB00539

Size:96 Wells

Human Cystatin B ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human Cystatin B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cystatin B has been precoated onto 96-well plates. Standards(E.coli, M2-F98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cystatin B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Cystatin B amount of sample captured in plate.
• ELISA Kit Range: 62.5 pg/ml - 4,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	421	1169	2311	428	1207	2365
  SD	26.1	67.8	140.97	30.82	82.08	165.6
  CV%	6.2	5.8	6.1	7.2	6.8	7

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human Cystatin B Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human Cystatin B Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human Cystatin B Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human Cystatin B
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: M2-F98
• Description: For quantitative detection of human Cystatin B in cell culture supernatants, serum, plasma(heparin, EDTA), saliva, urine and human milk.

Target Info

• Gene Symbol: CSTB
• Gene Full Name: cystatin B (stefin B)
• Alias Symbols: CHROW21, CPI B, CPI-B, CST6, CSTB, Cystatin B, Cystatin-B, CYTB, CYTB_HUMAN, EPM1, EPM1A, Liver thiol proteinase inhibitor, PME, Stefin-B, STFB, ULD
• NCBI Gene Id: 1476
• Protein Name: Cystatin-B
• Description of Target: Cystatin B (CSTB), also called STFB, is a small protein that is a member of the superfamily of cysteine protease inhibitors. It has been isolated from human spleen and liver and its amino acid sequence has been fully determined. The cystatin B gene is located on 21q22.3. It is widely distributed and is localized mostly intracellularly, but has been found extracellularly. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Its role is thought to be as a protector against the proteinases leaking from lysosomes. A cystatin B multiprotein complex might have a specific cerebellar function, and that the loss of this function might contribute to the etiopathogenesis of EPM1. Upon differentiation to myotubes, CSTB becomes excluded from the nucleus and lysosomes, suggesting that the subcellular distribution of CSTB is dependent on the differentiation status of the cell.
• Uniprot ID: P04080
• Protein Accession #: NP_000091.1
• Nucleotide Accession #: NM_000100.3