CRISP2 ELISA Kit (Human) (OKCD00460)

Catalog No: OKCD00460 (Formerly GWB-KBBJG6)

Size:96WELLS

Human CRISP2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Rich Secretory Protein 2 (CRISP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine Rich Secretory Protein 2 (CRISP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Rich Secretory Protein 2 (CRISP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cysteine Rich Secretory Protein 2 (CRISP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteine Rich Secretory Protein 2 (CRISP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteine Rich Secretory Protein 2 (CRISP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CRISP2 Microplate: 96 Wells (12 x 8 Well Strips)
  • CRISP2 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated CRISP2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cysteine Rich Secretory Protein 2 (CRISP2). No significant cross-reactivity or interference between Cysteine Rich Secretory Protein 2 (CRISP2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CRISP2
• Gene Full Name: cysteine rich secretory protein 2
• Alias Symbols: cancer/testis antigen 36;CRISP-2;CT36;cysteine-rich secretory protein 2;GAPDL5;glyceraldehyde-3-phosphate dehydrogenase-like 5;testicular tissue protein Li 43;testis specific protein 1 (probe H4-1 p3-1);testis-specific protein TPX-1;TPX1;TSP1.
• NCBI Gene Id: 7180
• Protein Name: Cysteine-rich secretory protein 2
• Description of Target: May regulate some ion channels' activity and therebye regulate calcium fluxes during sperm capacitation.
• Uniprot ID: P16562
• Protein Accession #: NP_001135879
• Nucleotide Accession #: NM_001142407.2
• Protein Size (# AA): 243