CITRATE SYNTHASE ELISA Kit (Human) (OKCD01175)

Catalog No: OKCD01175 (Formerly GWB-KBBRU2)

Size:96WELLS

Human Citrate synthase ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	81-91%	92-105%	80-95%	81-103%
  EDTA plasma(n=5)	83-95%	95-102%	85-94%	95-105%
  heparin plasma(n=5)	99-105%	85-94%	89-101%	82-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Citrate Synthase (CS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Citrate Synthase (CS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Citrate Synthase (CS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Citrate Synthase (CS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	96-104	99
  EDTA plasma(n=5)	84-102	94
  heparin plasma(n=5)	95-102	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Citrate Synthase (CS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Citrate Synthase (CS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Citrate synthase Microplate: 96 Wells (12 x 8 Well Strips)
  • Citrate synthase Lyophilized Standard: 2 x 400 ng
  • 100X Biotinylated Citrate synthase Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 2.39 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Citrate Synthase (CS). No significant cross-reactivity or interference between Citrate Synthase (CS) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CS
• Gene Full Name: citrate synthase
• Alias Symbols: citrate (Si)-synthase;citrate synthase, mitochondrial.
• NCBI Gene Id: 1431
• Protein Name: Citrate synthase, mitochondrial
• Description of Target: Citrate synthase, mitochondrial
• Uniprot ID: O75390
• Protein Accession #: NP_004068
• Nucleotide Accession #: NM_004077.2
• Protein Size (# AA): 466