CCL17 ELISA Kit (Mouse) : 96 Wells (OKCA00162)

Catalog No: OKCA00162

Size:96 Wells

Mouse CCL17 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Linearity:

  Sample Type 1
  Sample Type	Dilution	Average %	Range %
  Serum (n=4)	1:1	92%	80-102%
  Serum (n=4)	1:2	89%	82-103%
  Serum (n=4)	1:4	93%	89-110%
  Serum (n=4)	1:8	97%	88-110%

• ELISA Kit Principle: Aviva Systems Biology CCL17 ELISA Kit (Mouse) (OKCA00162) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mouse CCL17 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mouse CCL17 is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Mouse CCL17 captured in well.
• ELISA Kit Range: 31.25 pg/mL - 2,000 pg/mL
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	20	20	20	20	20	20
  Mean (pg/ml)	187.77	201.776	210.341	199.733	181.252	200.108
  SD	0.084	0.041	0.083	0.068	0.118	0.125
  CV%	6.541	3.025	5.837	5.036	9.478	9.218

• ELISA Kit Component:
  • Anti-CCL17 Microplate: 96 Well (12 x 8 Well Strips)
  • CCL17 Standard (Lyophilized): 2 x 2 ng
  • 100X CCL17 Detection Antibody: 1 x 120 uL
  • 100X HRP-Avidin: 1 x 120 uL
  • Detection Antibody Diluent: 1 x 10 mL
  • HRP-Avidin Diluent: 1 x 10 mL
  • Sample Diluent: 1 x 20 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell culture supernates and Tissue homogenates
• Sensitivity: < 10.512 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse TARC. No significant cross-reactivity or interference between mouse TARC and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CCL17
• Gene Full Name: chemokine (C-C motif) ligand 17
• Alias Symbols: TA, Scya, Tarc, ABCD-, Abcd-2, Scya17, Scya17l
• NCBI Gene Id: 20295
• Protein Name: C-C motif chemokine
• Uniprot ID: F6R5P4, Q9WUZ6
• Protein Accession #: NP_035462
• Nucleotide Accession #: XM_006530783
• Protein Size (# AA): 103
• Molecular Weight: 12 kDa