CA15-3 ELISA Kit (Human) : 96 Wells (OKCA00009)

Catalog No: OKCA00009

Size:96 Wells

Human CA15-3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Linearity:

  Sample Type 1
  Sample Type	Dilution	Average %	Range %
  Human Serum (n=4)	1:1	90%	86-99%
  Human Serum (n=4)	1:2	97%	91-105%
  Human Serum (n=4)	1:4	95%	89-100%
  Human Serum (n=4)	1:8	92%	88-98%

• ELISA Kit Principle: Aviva Systems Biology CA15-3 ELISA Kit (Human) (OKCA00009) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for human CA15-3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for human CA15-3 is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample human CA15-3 captured in well.
• ELISA Kit Range: 10 U/mL - 300 U/mL
• ELISA Kit Recovery:

  Sample 1
  Sample Type	Average Recovery	Range
  Serum (n=5)	97%	93-99%

  Sample 2
  Sample Type	Average Recovery	Range
  EDTA Plasma (n=4)	97%	90-101%

• ELISA Kit Reproducibility: Intra-assay: CV% < 15% (n = 20).Inter-assay: CV% < 20% (n = 20)
• ELISA Kit Component:

  Component	Amount
  Anti-CA15-3 Microplate	96 Well (12 x 8 Well Strips)
  CA15-3 Standard (Lyophilized)	4 x 0.7 ml
  HRP-conjugate	1 x 11 ml
  Sample Diluent	1 x 11 ml
  20X Wash Buffer	1 x 15 mL
  Substrate A	1 x 7 mL
  Substrate B	1 x 7 mL
  Stop Solution	1 x 7 mL

• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma
• Sensitivity: < 2.5 U/ml
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CA15-3. No significant cross-reactivity or interference between human CA15-3 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human CA15-3 and all the analogues, therefore, cross reaction may still exist.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CA15-3
• Gene Full Name: Mucin 1, cell surface associated
• Alias Symbols: EMA, MCD, PEM, PUM, KL-6, MAM6, MCKD, PEMT, CD227, H23AG, MCKD1, MUC-1, ADMCKD, ADTKD2, ADMCKD1, CA 15-3, MUC-1/X, MUC1/ZD, MUC-1/SEC
• NCBI Gene Id: 4582
• Protein Name: Mucin-1
• Description of Target: This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]
• Uniprot ID: P15941, Q7Z543, B6ECB0
• Protein Accession #: NP_001018016
• Nucleotide Accession #: NM_001018016
• Protein Size (# AA): 1255
• Molecular Weight: 122 kDa