B4GALT1 Antibody - C-terminal region (OAAB05488)

Catalog No: OAAB05488 (Formerly GWB-JWPGWW)

Size:400ul

Western blot analysis of B4GalT1 Antibody (C-term) in HL-60 cell lysates (35ug/lane). B4GALT1 (arrow) was detected using the purified Pab.

B4GALT1 is supported by BioGPS gene expression data to be expressed in HL60

B4GalT1 Antibody (C-term) (Cat. #OAAB05488) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, FC
• Additional Information: Function: The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
  Cellular Location: Isoform Long: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cell surface. Cell projection, filopodium. Note=Found in trans cisternae of Golgi but is mainly localized at the plasma membrane. B4GALT1 cell surface expression is regulated by UBE2Q1. Processed beta-1,4-galactosyltransferase 1: Secreted. Note=Soluble form found in body fluids.
  Tissue Location: Ubiquitously expressed, but at very low levels in fetal and adult brain.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This B4GalT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-358 amino acids from the C-terminal region of human B4GalT1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Bovine, Mouse
• Concentration: 0.45 mg/ml
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  FC: 1:10~50

Target Info

• Gene Symbol: B4GALT1
• Alias Symbols: GT1, GTB, CDG2D, GGTB2, B4GAL-T1, beta4Gal-T1
• NCBI Gene Id: 2683
• Protein Name: Beta-1,4-galactosyltransferase 1
• Description of Target: B4GalT1 is an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose UDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing.
  
• Uniprot ID: P15291
• Protein Accession #: NP_001488.2
• Molecular Weight: 44 kDa