ARHGDIB ELISA Kit (Human) (OKCD01160)

Catalog No: OKCD01160 (Formerly GWB-KBBRV6)

Size:96WELLS

Human ARHGDIB ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rho GDP Dissociation Inhibitor Beta (ARHGDIb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rho GDP Dissociation Inhibitor Beta (ARHGDIb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rho GDP Dissociation Inhibitor Beta (ARHGDIb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Rho GDP Dissociation Inhibitor Beta (ARHGDIb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rho GDP Dissociation Inhibitor Beta (ARHGDIb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rho GDP Dissociation Inhibitor Beta (ARHGDIb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ARHGDIB Microplate: 96 Wells (12 x 8 Well Strips)
  • ARHGDIB Lyophilized Standard: 2 x 40 ng
  • 100X Biotinylated ARHGDIB Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.241 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rho GDP Dissociation Inhibitor Beta (ARHGDIb). No significant cross-reactivity or interference between Rho GDP Dissociation Inhibitor Beta (ARHGDIb) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ARHGDIB
• Gene Full Name: Rho GDP dissociation inhibitor beta
• Alias Symbols: D4;GDIA2;GDID4;Ly-GDI;LYGDI;RAP1GN1;Rho GDI 2;Rho GDP dissociation inhibitor (GDI) beta;rho GDP-dissociation inhibitor 2;rho-GDI beta;RhoGDI2.
• NCBI Gene Id: 397
• Protein Name: Rho GDP-dissociation inhibitor 2
• Description of Target: Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:8356058, PubMed:7512369). Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133).
• Uniprot ID: P52566
• Protein Accession #: NP_001166
• Nucleotide Accession #: NM_001175.6
• Protein Size (# AA): 201