ADIPOQ ELISA Kit (Bovine) (OKEH03794)

Catalog No: OKEH03794

Size:96T

Bovine ADIPOQ ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ADIPOQ ELISA Kit (Bovine) (OKEH03794) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADIPOQ has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADIPOQ is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADIPOQ captured in well.
• ELISA Kit Range: 0.625-40ug/mL
• ELISA Kit Reproducibility: Mean Intra-Assay Precision - < 5.7%, n = 20
  Mean Inter-Assay Precision - < 8.4%, n = 20
• ELISA Kit Component:
  • Anti-ADIPOQ Microplate: 96 Wells (12 x 8 Well strips)
  • ADIPOQ Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated ADIPOQ Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.286ug/mL
• Specificity: Natural and recombinant Bovine Adiponectin
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ADIPOQ
• Gene Full Name: adiponectin, C1Q and collagen domain containing
• Alias Symbols: 30 kDa adipocyte complement-related protein;ACRP30;adipocyte complement related 30kDa protein;adipocyte complement related protein of 30 kDa;adipocyte complement-related 30 kDa protein;adipocyte, C1q and collagen domain-containing protein;adiponectin;adipose most abundant gene transcript 1 protein;apM-1;APM1.
• NCBI Gene Id: 282865
• Protein Name: Q3Y5Z3
• Description of Target: Adiponectin
• Uniprot ID: https://www.uniprot.org/uniprot/Q3Y5Z3
• Protein Accession #: NP_777167.1
• Nucleotide Accession #: NM_174742.2
• Protein Size (# AA): 240
• Molecular Weight: 26 kDa