Catalog No: OKCD02068 (Formerly GWB-KBBXX7)
Size:96WELLS
Price: $800.00
SKU
OKCD02068
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for Spi1 ELISA Kit (Rat) (OKCD02068) |
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Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.116 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1). No significant cross-reactivity or interference between Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | Spi1 |
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Gene Full Name | Spi-1 proto-oncogene |
Alias Symbols | hematopoietic transcription factor PU.1;Pu.1;SFFV proviral integration 1;Sfpi1;spleen focus forming virus (SFFV) proviral integration oncogene spi1;spleen focus forming virus proviral integration oncogene spi1;transcription factor PU.1. |
NCBI Gene Id | 366126 |
Protein Name | Transcription factor PU.1 |
Description of Target | Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity). |
Uniprot ID | Q6BDS1 |
Protein Accession # | NP_001005892.1 |
Nucleotide Accession # | NM_001005892.2 |
Protein Size (# AA) | 271 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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