Pla2g2d ELISA Kit (Mouse) (OKCD01564)

Catalog No: OKCD01564 (Formerly GWB-KBBMF4)

Size:96WELLS

Mouse Pla2g2d ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	91-103%	96-105%	84-101%	86-101%
  EDTA Plasma (n=5)	97-104%	79-92%	80-98%	80-95%
  Heparin Plasma (n=5)	94-101%	94-101%	98-105%	84-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group IID (PLA2G2D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group IID (PLA2G2D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group IID (PLA2G2D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Phospholipase A2, Group IID (PLA2G2D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	83-99	92
  EDTA Plasma (n=5)	95-104	101
  Heparin Plasma (n=5)	87-94	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase A2, Group IID (PLA2G2D) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase A2, Group IID (PLA2G2D) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Pla2g2d Microplate: 96 Wells (12 x 8 Well Strips)
  • Pla2g2d Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated Pla2g2d Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 13.8 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Group IID (PLA2G2D). No significant cross-reactivity or interference between Phospholipase A2, Group IID (PLA2G2D) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Pla2g2d
• Gene Full Name: phospholipase A2, group IID
• Alias Symbols: GIID;GIID sPLA2;group IID secretory phospholipase A2;mGI;mGIID;phosphatidylcholine 2-acylhydrolase 2D;PLA2IID;S;secretory-type PLA, stroma-associated homolog;sPLA2-IID;Splash.
• NCBI Gene Id: 18782
• Protein Name: Group IID secretory phospholipase A2
• Description of Target: PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. L-alpha-1-palmitoyl-2-linoleoyl phosphatidylethanolamine is more efficiently hydrolyzed than the other phospholipids examined.
• Uniprot ID: Q9WVF6
• Protein Accession #: NP_035239.1
• Nucleotide Accession #: NM_011109.2
• Protein Size (# AA): 144