Marcks ELISA Kit (Rat) (OKCD01968)

Catalog No: OKCD01968 (Formerly GWB-KBBJT2)

Size:96WELLS

Rat Marcks ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Marcks Microplate: 96 Wells (12 x 8 Well Strips)
  • Marcks Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated Marcks Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.55 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS). No significant cross-reactivity or interference between Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MARCKS
• Gene Full Name: Myristoylated alanine rich protein kinase C substrate
• Alias Symbols: Protein kinase C substrate 80 kDa protein.
• NCBI Gene Id: 25603
• Protein Name: Myristoylated alanine-rich C-kinase substrate
• Description of Target: MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
• Uniprot ID: P30009
• Protein Accession #: NP_001258019.1
• Nucleotide Accession #: NM_001271090.2
• Protein Size (# AA): 309