MMRN2 ELISA Kit (Human) (OKCD01875)

Catalog No: OKCD01875 (Formerly GWB-KBBBV6)

Size:96WELLS

Human MMRN2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	86-93%	97-105%	95-102%	96-104%
  EDTA Plasma (n=5)	84-94%	81-101%	94-103%	80-103%
  Heparin Plasma (n=5)	79-95%	82-94%	90-97%	89-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Multimerin 2 (MMRN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Multimerin 2 (MMRN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Multimerin 2 (MMRN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Multimerin 2 (MMRN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	79-101	84
  EDTA Plasma (n=5)	78-88	82
  Heparin Plasma (n=5)	79-103	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Multimerin 2 (MMRN2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Multimerin 2 (MMRN2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MMRN2 Microplate: 96 Wells (12 x 8 Well Strips)
  • MMRN2 Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated MMRN2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 28 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Multimerin 2 (MMRN2). No significant cross-reactivity or interference between Multimerin 2 (MMRN2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MMRN2
• Gene Full Name: multimerin 2
• Alias Symbols: elastin microfibril interface located protein 3;elastin microfibril interfacer 3;EMILIN3;EMILIN-3;EMILIN-like protein EndoGlyx-1;ENDOGLYX-1;endoGlyx-1 p125/p140 subunit;multimerin-2.
• NCBI Gene Id: 79812
• Protein Name: Multimerin-2
• Description of Target: Inhibits endothelial cells motility and acts as a negative regulator of angiogenesis; it downregulates KDR activation by binding VEGFA.
• Uniprot ID: Q9H8L6
• Protein Accession #: NP_079032.2
• Nucleotide Accession #: NM_024756.2
• Protein Size (# AA): 949