ITLN2 ELISA Kit (Human) (OKCD01969)

Catalog No: OKCD01969 (Formerly GWB-KBBBZ1)

Size:96WELLS

Human ITLN2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	81-98%	86-101%	83-95%	78-94%
  EDTA Plasma (n=5)	78-94%	79-97%	92-105%	90-99%
  Heparin Plasma (n=5)	84-98%	82-91%	90-104%	96-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Intelectin 2 (ITLN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Intelectin 2 (ITLN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Intelectin 2 (ITLN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Intelectin 2 (ITLN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	94-101	97
  EDTA Plasma (n=5)	88-104	99
  Heparin Plasma (n=5)	91-99	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intelectin 2 (ITLN2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intelectin 2 (ITLN2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ITLN2 Microplate: 96 Wells (12 x 8 Well Strips)
  • ITLN2 Lyophilized Standard: 2 x 200 ng
  • 100X Biotinylated ITLN2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 1.06 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Intelectin 2 (ITLN2). No significant cross-reactivity or interference between Intelectin 2 (ITLN2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ITLN2
• Gene Full Name: Intelectin 2
• Alias Symbols: HL2, HL-2
• NCBI Gene Id: 142683
• Protein Name: Intelectin-2
• Description of Target: May play a role in the defense system against pathogens.
• Uniprot ID: Q8WWU7
• Protein Accession #: NP_543154.1
• Nucleotide Accession #: NM_080878.2
• Protein Size (# AA): 325