Adora2b ELISA Kit (Mouse) (OKCD01893)

Catalog No: OKCD01893 (Formerly GWB-KBBLO8)

Size:96WELLS

Mouse Adora2b ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenosine A2b Receptor (ADORA2b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenosine A2b Receptor (ADORA2b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenosine A2b Receptor (ADORA2b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Adenosine A2b Receptor (ADORA2b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenosine A2b Receptor (ADORA2b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenosine A2b Receptor (ADORA2b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Adora2b Microplate: 96 Wells (12 x 8 Well Strips)
  • Adora2b Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated Adora2b Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 30 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adenosine A2b Receptor (ADORA2b). No significant cross-reactivity or interference between Adenosine A2b Receptor (ADORA2b) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Adora2b
• Gene Full Name: adenosine A2b receptor
• Alias Symbols: A2;A2b;A2b, Rs;A2BAR;A2BR;AA;AA2BR;adenosine receptor A2b;AI480866;AI605384;AR;ARA2B.
• NCBI Gene Id: 11541
• Protein Name: Adenosine receptor A2b
• Description of Target: Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
• Uniprot ID: Q60614
• Protein Accession #: NP_031439.2
• Nucleotide Accession #: NM_007413.4
• Protein Size (# AA): 332