NFATC1 Antibody - N-terminal region (P100981_P050)

• Product Number: P100981_P050
• Name: NFATC1 Antibody - N-terminal region (P100981_P050)
• Protein Size (# AA): 716 amino acids
• Molecular Weight: 78kDa
• NCBI Gene Id: 4772
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
• Description:
  View product citations for NFATC1 Antibody - N-terminal region (P100981_P050)on CiteAb
• Alias Symbols: NFAT2, NFATc, NF-ATC, NF-ATc1.2
• Peptide Sequence: Synthetic peptide located within the following region: MPSTSFPVPSKFPLGPAAAVFGRGETLGPAPRAGGTMKSAEEEHYGYASS
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Crotti,T.N., (2008) J. Cell. Physiol. 215 (3), 636-644
• Description of Target: NFATC1 is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Different isoforms of this protein may regulate inducible expression of different cytokine genes. The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.
• Protein Interactions: YWHAQ; OGT; PML; GZF1; FOXP3; HDAC4; PIAS1; SUMO1; UBE2I; RPS6KB1; RPS6KA3; HDAC2; HDAC1; HNRNPA1; TUBA1A; KPNB1; HDAC5; KAT2B; EP300; PARP1; HDAC3; PPP3R1; MAPK14; GNB2L1; PRKCA; SPI1; PIM1; EGR2; EGR1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-NFATC1 (P100981_P050) antibody is Catalog # AAP31109 (Previous Catalog # AAPP01848)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NFATC1
• Uniprot ID: O95644-2
• Protein Name: Nuclear factor of activated T-cells, cytoplasmic 1
• Protein Accession #: NP_765978
• Purification: Affinity Purified
• Nucleotide Accession #: NM_172390
• Tested Species Reactivity: Human
• Gene Symbol: NFATC1
• Predicted Species Reactivity: Human, Mouse, Dog, Guinea Pig
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Dog: 91%; Guinea Pig: 84%; Human: 100%; Mouse: 91%
• Image 1: Human Stomach
  
  WB Suggested Anti-NFATC1 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:312500
  Positive Control: Human Stomach
• Image 2: liver
  
  Rabbit Anti-NFATC1 Antibody
  Catalog Number: P100981_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Adult liver
  Observed Staining: Cytoplasmic in bile ducts and in sinusoids not in hepatocytes
  Primary Antibody Concentration: 1:600
  Secondary Antibody: Donkey anti-Rabbit-Cy2/3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 – 2.0 sec
  Protocol located in Reviews and Data.
• Image 3: Human liver
  
  WB Suggested Anti-NFATC1 antibody Titration: 1 ug/mL
  Sample Type: Human liver