HSPA8 Antibody - N-terminal region (ARP48445_P050)

• Product Number: ARP48445_P050
• Name: HSPA8 Antibody - N-terminal region (ARP48445_P050)
• Protein Size (# AA): 646 amino acids
• Molecular Weight: 71kDa
• NCBI Gene Id: 3312
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Heat shock 70kDa protein 8
• Description:
  View product citations for HSPA8 Antibody - N-terminal region (ARP48445_P050) on CiteAb
• Alias Symbols: LAP1, HSC54, HSC70, HSC71, HSP71, HSP73, LAP-1, NIP71, HEL-33, HSPA10, HEL-S-72p
• Peptide Sequence: Synthetic peptide located within the following region: MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERL
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Luciano,M., (er) Eur. J. Hum. Genet. (2008) In press
• Description of Target: HSPA8 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 is a heat-shock cognate protein. The protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date.The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date.
• Protein Interactions: FUS; HSPBP1; TRIM38; STUB1; ISG15; UBC; TP53; BAG1; BAG3; IL32; NMI; AIRE; HAUS2; TUBGCP4; CEP250; TUBGCP2; TUBGCP3; TUBG1; AURKA; SUMO2; SUMO3; MDM2; LGALS3BP; CDKN1A; CDC20; HAUS1; STAU1; ST13; HSPA4; HSPA2; DNAJA1; GABRB1; EDRF1; DNAJC9; HSPA4L; HSPH1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-HSPA8 (ARP48445_P050) antibody is Catalog # AAP48445 (Previous Catalog # AAPY01867)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HSPA8
• Uniprot ID: P11142
• Protein Name: Heat shock cognate 71 kDa protein
• Publications:

  Hsc70 Ameliorates the Vesicle Recycling Defects Caused by Excess a-Synuclein at Synapses. Eneuro. 7, (2020). 31941659

• Sample Type Confirmation:

  HSPA8 is strongly supported by BioGPS gene expression data to be expressed in Jurkat, MCF7

• Protein Accession #: NP_006588
• Purification: Affinity Purified
• Nucleotide Accession #: NM_006597
• Tested Species Reactivity: Human, Mouse, Rat
• Gene Symbol: HSPA8
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Goat, Pig, Sheep, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Goat: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100%
• Image 1: Rat Brain
  
  HSPA8 antibody - N-terminal region (ARP48445_P050) validated by WB using Rat Brain lysate at 1:1,000.
• Image 2: Mouse brain
  
  Sample Type: Mouse Brain Slices 
  Red: primary
  Blue: DAPI
  Primary
  Dilution: 1:400 
  Secondary Antibody: Anti-Rabbit IgG Alexa 594
  Secondary
  Dilution: 1:400 
  Image Submitted By: Adahir Labrador-Garrido and Cintia Roodveldt
  University of Seville
• Image 3: Human Jurkat Whole Cell
  
  Host: Rabbit
  Target Name: HSPA8
  Sample Tissue: Human Jurkat Whole Cell
  Antibody Dilution: 1ug/ml
• Image 4: Human Fetal Liver
  
  Host: Rabbit
  Target Name: HSPA8
  Sample Tissue: Human Fetal Liver
  Antibody Dilution: 1.0ug/ml
• Image 5: Human Pineal Tissue
  
  Rabbit Anti-HSPA8 Antibody
  Catalog Number: ARP48445_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue
  Observed Staining: Nuclear in pinealocytes
  Primary Antibody Concentration: 1:100
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
  
• Image 6:
  
  25 ug of the indicated Human whole cell tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.