MITF Antibody - middle region (ARP37978_P050)

• Product Number: ARP37978_P050
• Name: MITF Antibody - middle region (ARP37978_P050)
• Protein Size (# AA): 419 amino acids
• Molecular Weight: 47kDa
• NCBI Gene Id: 4286
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Microphthalmia-associated transcription factor
• Alias Symbols: MI, WS2, CMM8, WS2A, COMMAD, bHLHe32
• Peptide Sequence: Synthetic peptide located within the following region: MGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSMSMEETEHTC
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Chiaverini,C., (2008) J. Biol. Chem. 283 (18), 12635-12642
• Description of Target: MITF is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Protein Interactions: EBP; STUB1; HK3; EPAS1; LEF1; APP; HINT1; ELAVL1; CDK2; SUMO2; PSMD14; MARK3; PIAS3; PIAS2; PIAS1; SUMO1; UBE2I; PAX3; OTX2; PAX6; PATZ1; GSK3B; TFEC; EP300; RPS6KA1; TFEB; MAPK14; TFE3; MAPK1; SPI1; FOS; CTNNB1; CDKN1A; RB1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-MITF (ARP37978_P050) antibody is Catalog # AAP37978 (Previous Catalog # AAPP20081)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MITF
• Uniprot ID: O75030-9
• Protein Name: Microphthalmia-associated transcription factor
• Publications:

  BDNF rs6265 methylation and genotype interact on risk for schizophrenia. Epigenetics. 11, 11-23 (2016). 26889735

• Sample Type Confirmation:

  MITF is supported by BioGPS gene expression data to be expressed in HeLa

• Protein Accession #: NP_000239
• Purification: Affinity Purified
• Nucleotide Accession #: NM_000248
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: MITF
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100%
• Image 1: Mouse Liver
  
  Host: Mouse
  Target Name: MITF
  Sample Tissue: Mouse Liver
  Antibody Dilution: 1ug/ml
• Image 2: Human HeLa
  
  WB Suggested Anti-MITF Antibody Titration: 0.2-1 ug/ml
  Positive Control: Hela cell lysateMITF is supported by BioGPS gene expression data to be expressed in HeLa