CD8A Antibody : APC (OATA00027)

• Product Number: OATA00027
• Name: CD8A Antibody : APC (OATA00027)
• Clone: RPA-T8
• Isotype: Mouse IgG1, kappa
• Conjugation: APC: Allophycocyanin
• NCBI Gene Id: 925
• Host: Mouse
• Clonality: Monoclonal
• Gene Full Name: CD8a molecule
• Alias Symbols: CD8, p32, Leu2
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Reference:

  Estes JD, Gordon SN, Zeng M, Chahroudi AM, Dunham RM, Staprans SI, Reilly CS, Silvestri G, and Haase AT. 2008. J. Immunol. 180: 6798-6807. (Flow cytometry - Rhesus macaque and Sooty Mangabey)

  Chlereth B, Fichtner I, Lorenczewski G, Kleindienst P, Brischwein K, da Silva A, Kufer P, Lutterbuese R, Junghahn I, Kasimir-Bauer S, Wimberger P, Kimmig R and Baeuerle PA. 2005. Cancer Res. 65: 2882-2889. (Immunohistochemistry - frozen tissue)

  Mack CL, Tucker RM, Sokol RJ, Darrer FM, Kotzin BL, Whitington PF and Miller SD. 2004. Pediatr. Res. 56(1)

  :79-87. (Immunohistochemistry - frozen tissue)

  Huang Z-Y, Hunter S, Kim M-K, Chien P, Worth RG, Indik ZK, and Schreiber AD. 2004. J. Leukoc. Biol. 76:491-499. (in vitro activation)

  Kayagaki N, Yamaguchi N, Nagao F, Matsuo S, Maeda H, Okumura K, and Yagita H. 1997. Proc. Natl. Acad. Sci. 94:3914-3919. (Immunoprecipitation - transfected cells)

  Deng MC, Bell S, Huie P, Pinto F, Hunt SA, Stinson EB, Sibley R, Hall BM, and Valantine HA. 1995. Circulation. 91: 1647-1654. (Immunohistochemistry - OCT embedded frozen tissue)

• Description of Target: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene.
• Reconstitution and Storage: 2-8C protected from light
• Specificity: CD8a
• Additional Information: Excitation Laser: Red (633-640 nm)
• Application Info: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.
• Additional Information: Emmision: 660 nm
• Uniprot ID: P01732
• Protein Name: T-cell surface glycoprotein CD8 alpha chain
• Protein Accession #: NP_001139345.1?
• Nucleotide Accession #: NM_001145873.1
• Tested Species Reactivity: Human
• Gene Symbol: CD8A
• Predicted Species Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Sooty Mangabey
• Application: FC
• Image 1: Human peripheral blood lymphocytes
  
  Human peripheral blood lymphocytes were stained with 5 uL (0.25 ug) APC Anti-Human CD8a (OATA00027) (solid line) or 0.25 ug APC Mouse IgG1 isotype control (dashed line).