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CD8A Antibody : APC (OATA00027)

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Tested Species Reactivity:
Human
Predicted Species Reactivity:
Chimpanzee, Baboon, Cynomolgus, Rhesus, Sooty Mangabey
Product Format:
Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Clonality:
Monoclonal
Clone:
RPA-T8
Isotype:
Mouse IgG1, kappa
Host:
Mouse
Conjugation:
APC: Allophycocyanin
Application:
FC
:::
Excitation Laser: Red (633-640 nm)
:::
Emmision: 660 nm
Reconstitution and Storage:
2-8C protected from light
Gene Symbol:
CD8A
NCBI Gene Id:
925
Official Gene Full Name:
CD8a molecule
Protein Name:
T-cell surface glycoprotein CD8 alpha chain
Swissprot Id:
P01732
Protein Accession #:
NP_001139345.1
Nucleotide Accession #:
NM_001145873.1
Alias Symbols:
CD8, p32, Leu2
Description of Target:
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene.
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CD8A.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CD8A.
Datasheets/Manuals:
Printable datasheet for OATA00027
Specificity:
CD8a
Application Info:
This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.
Target Reference:

Estes JD, Gordon SN, Zeng M, Chahroudi AM, Dunham RM, Staprans SI, Reilly CS, Silvestri G, and Haase AT. 2008. J. Immunol. 180: 6798-6807. (Flow cytometry - Rhesus macaque and Sooty Mangabey)

Chlereth B, Fichtner I, Lorenczewski G, Kleindienst P, Brischwein K, da Silva A, Kufer P, Lutterbuese R, Junghahn I, Kasimir-Bauer S, Wimberger P, Kimmig R and Baeuerle PA. 2005. Cancer Res. 65: 2882-2889. (Immunohistochemistry - frozen tissue)

Mack CL, Tucker RM, Sokol RJ, Darrer FM, Kotzin BL, Whitington PF and Miller SD. 2004. Pediatr. Res. 56(1)

:79-87. (Immunohistochemistry - frozen tissue)

Huang Z-Y, Hunter S, Kim M-K, Chien P, Worth RG, Indik ZK, and Schreiber AD. 2004. J. Leukoc. Biol. 76:491-499. (in vitro activation)

Kayagaki N, Yamaguchi N, Nagao F, Matsuo S, Maeda H, Okumura K, and Yagita H. 1997. Proc. Natl. Acad. Sci. 94:3914-3919. (Immunoprecipitation - transfected cells)

Deng MC, Bell S, Huie P, Pinto F, Hunt SA, Stinson EB, Sibley R, Hall BM, and Valantine HA. 1995. Circulation. 91: 1647-1654. (Immunohistochemistry - OCT embedded frozen tissue)

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