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CD8A Antibody : APC (OATA00027)

Catalog#: OATA00027
Domestic: within 1 week delivery | International: 1 week
More Information
Tested Species Reactivity Human
Predicted Species Reactivity Chimpanzee, Baboon, Cynomolgus, Rhesus, Sooty Mangabey
Product Format Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Clonality Monoclonal
Clone RPA-T8
Isotype Mouse IgG1, kappa
Host Mouse
Conjugation APC: Allophycocyanin
Application FC
:: Excitation Laser: Red (633-640 nm)
:: Emmision: 660 nm
Reconstitution and Storage 2-8C protected from light
Datasheets/Manuals Printable datasheet for OATA00027
Specificity CD8a
Application Info This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.
Target Reference

Estes JD, Gordon SN, Zeng M, Chahroudi AM, Dunham RM, Staprans SI, Reilly CS, Silvestri G, and Haase AT. 2008. J. Immunol. 180: 6798-6807. (Flow cytometry - Rhesus macaque and Sooty Mangabey)

Chlereth B, Fichtner I, Lorenczewski G, Kleindienst P, Brischwein K, da Silva A, Kufer P, Lutterbuese R, Junghahn I, Kasimir-Bauer S, Wimberger P, Kimmig R and Baeuerle PA. 2005. Cancer Res. 65: 2882-2889. (Immunohistochemistry - frozen tissue)

Mack CL, Tucker RM, Sokol RJ, Darrer FM, Kotzin BL, Whitington PF and Miller SD. 2004. Pediatr. Res. 56(1)

:79-87. (Immunohistochemistry - frozen tissue)

Huang Z-Y, Hunter S, Kim M-K, Chien P, Worth RG, Indik ZK, and Schreiber AD. 2004. J. Leukoc. Biol. 76:491-499. (in vitro activation)

Kayagaki N, Yamaguchi N, Nagao F, Matsuo S, Maeda H, Okumura K, and Yagita H. 1997. Proc. Natl. Acad. Sci. 94:3914-3919. (Immunoprecipitation - transfected cells)

Deng MC, Bell S, Huie P, Pinto F, Hunt SA, Stinson EB, Sibley R, Hall BM, and Valantine HA. 1995. Circulation. 91: 1647-1654. (Immunohistochemistry - OCT embedded frozen tissue)

Gene Symbol CD8A
Official Gene Full Name CD8a molecule
Alias Symbols CD8, p32, Leu2
NCBI Gene Id 925
Protein Name T-cell surface glycoprotein CD8 alpha chain
Description of Target The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene.
Swissprot Id P01732
Protein Accession # NP_001139345.1
Nucleotide Accession # NM_001145873.1
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express CD8A.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express CD8A.
  1. What is the species homology for "CD8A Antibody : APC (OATA00027)"?

    The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Chimpanzee, Baboon, Cynomolgus, Rhesus, Sooty Mangabey".

  2. How long will it take to receive "CD8A Antibody : APC (OATA00027)"?

    This item is available "Domestic: within 1 week delivery | International: 1 week".

  3. What buffer format is "CD8A Antibody : APC (OATA00027)" provided in?

    This item is provided in "Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "CD8A Antibody : APC (OATA00027)"?

    This target may also be called "CD8, p32, Leu2" in publications.

  5. What is the shipping cost for "CD8A Antibody : APC (OATA00027)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "CD8A Antibody : APC (OATA00027)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "CD8A Antibody : APC (OATA00027)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "CD8A Antibody : APC (OATA00027)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "CD8A"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "CD8A"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "CD8A"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "CD8A"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "CD8A"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "CD8A"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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