Product Number |
OASG02995 |
Product Page |
www.avivasysbio.com/gclc-antibody-middle-region-oasg02995.html |
Name |
GCLC Antibody - middle region (OASG02995) |
Molecular Weight |
73 kDa |
Isotype |
IgG |
NCBI Gene Id |
2729; 14629; 25283 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1 mg/ml |
Gene Full Name |
glutamate-cysteine ligase, catalytic subunit |
Alias Symbols |
GCL, GCS, GLCL, GLCLC |
Product Format |
Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Description of Target |
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction. |
Reconstitution and Storage |
Store at -20C. Avoid repeated freeze/thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-GCLC (OASG02995) antibody |
Specificity |
GCSc-Gamma Polyclonal Antibody detects endogenous levels of GCSc-Gamma protein. |
Additional Information |
Function: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. Feedback inhibition by glutathione. Sequence & Domain Family: Belongs to the glutamate--cysteine ligase type 3 family. |
Application Info |
WB: 1:500~2000 ELISA: 1:10000 |
Immunogen |
Synthesized peptide derived from the Internal region of human GCSc-Gamma. |
Uniprot ID |
P48506, P97494, P19468 |
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Gene Symbol |
GCLC |
Predicted Species Reactivity |
Human, Mouse, Rat |
Application |
WB |
Image 1 | A549
| Western Blot using A549 |
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