GCLC Antibody - middle region (OASG02995)

Catalog#: OASG02995

• Size: 100 uL

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, ELISA
  Function: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. Feedback inhibition by glutathione.
  Sequence & Domain Family: Belongs to the glutamate--cysteine ligase type 3 family.
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Gene Symbol: GCLC
• Official Gene Full Name: glutamate-cysteine ligase, catalytic subunit
• NCBI Gene Id: 2729; 14629; 25283
• Swissprot Id: P48506, P97494, P19468
• Alias Symbols: GCLC, GLCL, GLCLC, Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase
• Replacement Item: This antibody may replace item sc-115522 from Santa Cruz Biotechnology.
• Description of Target: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
• Molecular Weight: 73 kDa
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express GCLC.
• Immunogen: Synthesized peptide derived from the Internal region of human GCSc-Gamma.
• Concentration: 1 mg/ml
• Specificity: GCSc-Gamma Polyclonal Antibody detects endogenous levels of GCSc-Gamma protein.
• Application Info: WB: 1:500~2000
  ELISA: 1:10000

Protocol:

Tips Information: