- Predicted Species Reactivity:
- Human, Mouse, Rat
- Product Format:
- Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Host:
- Rabbit
- Application:
- WB, ELISA
- :::
- Function: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. Feedback inhibition by glutathione.
Sequence & Domain Family: Belongs to the glutamate--cysteine ligase type 3 family.
- Reconstitution and Storage:
- Store at -20C. Avoid repeated freeze/thaw cycles.
- Official Gene Full Name:
- glutamate-cysteine ligase, catalytic subunit
- NCBI Gene Id:
- 2729; 14629; 25283
- Alias Symbols:
- GCLC, GLCL, GLCLC, Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase
- Replacement Item:
- This antibody may replace item sc-115522 from Santa Cruz Biotechnology.
- Description of Target:
- Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
- Molecular Weight:
- 73 kDa
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express GCLC.

- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express GCLC.

- Immunogen:
- Synthesized peptide derived from the Internal region of human GCSc-Gamma.
- Concentration:
- 1 mg/ml
- Specificity:
- GCSc-Gamma Polyclonal Antibody detects endogenous levels of GCSc-Gamma protein.