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GCLC Antibody - middle region (OASG02995)
| Predicted Species Reactivity | Human, Mouse, Rat |
|---|---|
| Product Format | Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Application | WB |
| :: | Function: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. Feedback inhibition by glutathione. Sequence & Domain Family: Belongs to the glutamate--cysteine ligase type 3 family. |
| Reconstitution and Storage | Store at -20C. Avoid repeated freeze/thaw cycles. |
| Replacement Item | This antibody may replace item sc-115522 from Santa Cruz Biotechnology. |
| Immunogen | Synthesized peptide derived from the Internal region of human GCSc-Gamma. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Datasheets/Manuals | Printable datasheet for anti-GCLC (OASG02995) antibody |
| Specificity | GCSc-Gamma Polyclonal Antibody detects endogenous levels of GCSc-Gamma protein. |
| Application Info | WB: 1:500~2000 ELISA: 1:10000 |
| Gene Symbol | GCLC |
|---|---|
| Official Gene Full Name | glutamate-cysteine ligase, catalytic subunit |
| Alias Symbols | GCLC, GLCL, GLCLC, Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase |
| NCBI Gene Id | 2729; 14629; 25283 |
| Description of Target | Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction. |
| Swissprot Id | P48506, P97494, P19468 |
| Molecular Weight | 73 kDa |
| Tissue Tool | Find tissues and cell lines supported by DNA array analysis to express GCLC.  |
| RNA Seq | Find tissues and cell lines supported by RNA-seq analysis to express GCLC.  |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "GCLC Antibody - middle region (OASG02995)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "GCLC Antibody - middle region (OASG02995)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
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What buffer format is "GCLC Antibody - middle region (OASG02995)" provided in?
This item is provided in "Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "GCLC Antibody - middle region (OASG02995)"?
This target may also be called "GCLC, GLCL, GLCLC, Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase" in publications.
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What is the shipping cost for "GCLC Antibody - middle region (OASG02995)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "GCLC Antibody - middle region (OASG02995)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "GCLC Antibody - middle region (OASG02995)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "73 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "GCLC Antibody - middle region (OASG02995)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.
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What are positive controls for "GCLC"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "GCLC"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "GCLC"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "GCLC"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "GCLC"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "GCLC"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
