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GCLC Antibody - middle region (OASG02995)

100 uL
$215.00
In Stock
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Predicted Species Reactivity:
Human, Mouse, Rat
Product Format:
Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality:
Polyclonal
Isotype:
IgG
Host:
Rabbit
Application:
WB, ELISA
:::
Function: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. Feedback inhibition by glutathione.
Sequence & Domain Family: Belongs to the glutamate--cysteine ligase type 3 family.
Reconstitution and Storage:
Store at -20C. Avoid repeated freeze/thaw cycles.
Gene Symbol:
GCLC
Official Gene Full Name:
glutamate-cysteine ligase, catalytic subunit
NCBI Gene Id:
2729; 14629; 25283
Swissprot Id:
P48506, P97494, P19468
Alias Symbols:
GCLC, GLCL, GLCLC, Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase
Replacement Item:
This antibody may replace item sc-115522 from Santa Cruz Biotechnology.
Description of Target:
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
Molecular Weight:
73 kDa
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GCLC.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GCLC.
Immunogen:
Synthesized peptide derived from the Internal region of human GCSc-Gamma.
Concentration:
1 mg/ml
Datasheets/Manuals:
Printable datasheet for anti-GCLC (OASG02995) antibody
Specificity:
GCSc-Gamma Polyclonal Antibody detects endogenous levels of GCSc-Gamma protein.
Application Info:
WB: 1:500~2000
ELISA: 1:10000

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