| Product Number |
OASA00349 |
| Product Page |
www.avivasysbio.com/il12a-antibody-oasa00349.html |
| Name |
IL12A Antibody (OASA00349) |
| Protein Size (# AA) |
221 amino acids |
| Clone |
CC301 |
| Isotype |
IgG2a |
| NCBI Gene Id |
281856 |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Concentration |
1 mg/ml |
| Alias Symbols |
IL12p35, Il-12 p35 |
| Product Format |
Liquid. Phosphate buffered saline |
| Reference |
1. Hope, J. C. et al. (2002) Development of detection methods for ruminant interleukin (IL) - 12. J. Immunol. Methods. 266: 117 - 126.
2. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis. J Dairy Sci. 93: 2458-70.
3. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis. Funct Integr Genomics. 10: 21-38.
|
| Description of Target |
MOUSE ANTI BOVINE INTERLEUKIN-12:Low Endotoxin
|
| Reconstitution and Storage |
-20°C |
| Datasheets/Manuals |
Printable datasheet for IL12A Antibody (OASA00349) |
| Specificity |
Mouse anti Bovine Interleukin-12/23 antibody, clone CC301 recognizes the p40 subunit of bovine interleukin-12, and binds to both the free subunit and the intact heterodimer. The p40 subunit is also known as IL-12B and can form a heterodimer with either IL-12A or IL-23A.
DOES NOT REACT WITH MOUSE |
| Additional Information |
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Endotoxin Level: < 0.01 EU/ug |
| Application Info |
ELISA: 8ug/ml
ELISA : This antibody may be used as a capture antibody in a sandwich ELISA in combination with biotinylated clone CC326 (OASA00348) as detection reagent.
Functional Assay: 1/100
Western Blot: 1/50-1/100
Flow Cytometry*: 1/10
*Membrane permeabilisation is required for this application
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Antibody reactivity and working conditions may vary between species.Product Form
|
| Immunogen |
Recombinant bovine IL-12. |
| Uniprot ID |
P54349 |
| Protein Name |
Interleukin-12 subunit alpha |
| Protein Accession # |
NP_776780.1 |
| Purification |
Protein A Purified |
| Gene Symbol |
IL12A |
| Predicted Species Reactivity |
Bovine |
| Application |
Enzyme-linked immunosorbent assay|Enzyme-linked immunosorbent spot assay|Flow cytometry|Functional assay|Western blot |
| Image 1 | |
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