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- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for IL12A Antibody (OASA00349) |
|---|
| Predicted Species Reactivity | Bovine |
|---|---|
| Product Format | Liquid. Phosphate buffered saline |
| Clonality | Monoclonal |
| Clone | CC301 |
| Isotype | IgG2a |
| Host | Mouse |
| Application | Enzyme-linked immunosorbent assay|Enzyme-linked immunosorbent spot assay|Flow cytometry|Functional assay|Western blot |
| Additional Information | Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line. Endotoxin Level: < 0.01 EU/ug |
| Reconstitution and Storage | -20°C |
| Immunogen | Recombinant bovine IL-12. |
| Purification | Protein A Purified |
| Concentration | 1 mg/ml |
| Specificity | Mouse anti Bovine Interleukin-12/23 antibody, clone CC301 recognizes the p40 subunit of bovine interleukin-12, and binds to both the free subunit and the intact heterodimer. The p40 subunit is also known as IL-12B and can form a heterodimer with either IL-12A or IL-23A. DOES NOT REACT WITH MOUSE |
| Application Info | ELISA: 8ug/ml ELISA : This antibody may be used as a capture antibody in a sandwich ELISA in combination with biotinylated clone CC326 (OASA00348) as detection reagent. Functional Assay: 1/100 Western Blot: 1/50-1/100 Flow Cytometry*: 1/10 *Membrane permeabilisation is required for this application Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Antibody reactivity and working conditions may vary between species.Product Form |
| Reference | 1. Hope, J. C. et al. (2002) Development of detection methods for ruminant interleukin (IL) - 12. J. Immunol. Methods. 266: 117 - 126. 2. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis. J Dairy Sci. 93: 2458-70. 3. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis. Funct Integr Genomics. 10: 21-38. |
| Gene Symbol | IL12A |
|---|---|
| Alias Symbols | IL12p35, Il-12 p35 |
| NCBI Gene Id | 281856 |
| Protein Name | Interleukin-12 subunit alpha |
| Description of Target | MOUSE ANTI BOVINE INTERLEUKIN-12:Low Endotoxin |
| Uniprot ID | P54349 |
| Protein Accession # | NP_776780.1 |
| Protein Size (# AA) | 221 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "IL12A Antibody (OASA00349)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine".
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How long will it take to receive "IL12A Antibody (OASA00349)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "IL12A Antibody (OASA00349)" provided in?
This item is provided in "Liquid. Phosphate buffered saline".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "IL12A Antibody (OASA00349)"?
This target may also be called "IL12p35, Il-12 p35" in publications.
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What is the shipping cost for "IL12A Antibody (OASA00349)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "IL12A Antibody (OASA00349)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "IL12A Antibody (OASA00349)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "IL12A Antibody (OASA00349)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "IL12A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "IL12A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "IL12A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "IL12A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "IL12A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "IL12A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
