Anti-Tyrosine Hydroxylase (OAPC00077)

• Product Number: OAPC00077
• Name: Anti-Tyrosine Hydroxylase (OAPC00077)
• Molecular Weight: ~60kDa
• Quality Control: Dot blots and Western blots performed with each lot
• NCBI Gene Id: 25085
• Host: Sheep
• Clonality: Polyclonal
• Concentration: 0.15 mg/ml
• Alias Symbols: The
• Product Format: Liquid10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol. Adequate amount of material to conduct 10 mini Western blots.
• Reference: Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.
  Salvatore MF, Waymire JC, Haycock JW (2001) Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J Neurochem 79:349-360.
  Witkovsky P, Gabriel R, Haycock JW, Meller E (2000) Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J Chem Neuroanat 19:105-116.
  Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.
  Zhu MY, Klimek V, Dilley GE, Haycock JW, Stockmeier C, Overholser JC, Meltzer HY, Ordway GA (1999) Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286.
• Description of Target: Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001).
• Reconstitution and Storage: For long-term storage, -80C is recommended, but shorter-term storage at -20C is also acceptable as aliquots may be taken without freezing/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of one (1) year at -20C.
• Specificity: Specific for the ~60k tyrosine hydroxylase protein in Western blots of rat brain lysates and PC-12 cells stimulated by Okadaic acid. The antibody cross-reacts with all mammalian and at least some non-mammalian forms of the enzyme in WB and IF/IHC.
• Application Info: DB: 1:1000
  IF: 1:1000
  IHC: 1:1000
  WB: 1:1000
• Uniprot ID: P04177
• Protein Name: Tyrosine 3-monooxygenase
• Protein Accession #: NP_036872.1
• Purification: Affinity purified
• Gene Symbol: TH
• Predicted Species Reactivity: Rat, Most mammals, Some invertebrates
• Application: DB, IF, IHC, WB
• Predicted Homology Based on Immunogen Sequence: Most mammals, Some invertebrates
• Image 1: Rabbit retina
  
  Immunostaining of rabbit retina showing specific labeling of tyrosine hydroxylase in green.
• Image 2: Rat caudate
  
  Western blot of 10 ug of rat caudate lysate showing specific immunolabeling of the ~ 60 k tyrosine hydroxylase proteins.