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Anti-Tyrosine Hydroxylase (OAPC00077)

100 ul
$349.00
In Stock
Gene Symbol:
TH
NCBI Gene Id:
25085
Protein Name:
Tyrosine 3-monooxygenase
Swissprot Id:
P04177
Protein Accession #:
NP_036872.1
Replacement Item:
This antibody may replace item sc-136100 from Santa Cruz Biotechnology.
Description of Target:
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001).
Molecular Weight:
~60kDa
Host:
Sheep
Clonality:
Polyclonal
Purification:
Prepared from sheep serum by affinity purification using a column to which immunogen was coupled. The antibody is predominantly of the IgG1 subclass.
Application:
WB, IF, IHC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express TH.
RNA Seq:
Find tissues and cell lines supported by DNA array analysis to express TH.
Immunogen:
Native rat tyrosine hydroxylase, purified from pheochromocytoma.
Species Reactivity:
Most mammals, Some invertebrates
Predicted Homology Based on Immunogen Sequence:
Most mammals, Some invertebrates
Product Format:
Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Reconstitution and Storage:
For long term storage -20C is recommended. Stable at -20C for at least 1 year.
Datasheets/Manuals:
Printable datasheet for OAPC00077
Specificity:
Specific for the ~60k tyrosine hydroxylase protein.
Application Info:
The antibody recognizes all mammalian and at least some non-mammalian forms of the enzyme in Western blots and in IHC/IF.
Immunostaining of rabbit retina showing specific labeling of tyrosine hydroxylase in green.
Western blot of 10 ug of rat caudate lysate showing specific immunolabeling of the ~ 60 k tyrosine hydroxylase proteins.
Additional Information:
Packaging :
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Target Reference:
Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.
Salvatore MF, Waymire JC, Haycock JW (2001) Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J Neurochem 79:349-360.
Witkovsky P, Gabriel R, Haycock JW, Meller E (2000) Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J Chem Neuroanat 19:105-116.
Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.
Zhu MY, Klimek V, Dilley GE, Haycock JW, Stockmeier C, Overholser JC, Meltzer HY, Ordway GA (1999) Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286.
Protocol:
Tips Information:

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