RAD51 Antibody (OAGA08778)

• Product Number: OAGA08778
• Name: RAD51 Antibody (OAGA08778)
• Molecular Weight: 37 kDa
• Clone: 14B4
• Isotype: IgG2b
• NCBI Gene Id: 5888
• Host: Mouse
• Clonality: Monoclonal
• Concentration: 1.94 mg/ml
• Gene Full Name: RAD51 recombinase
• Alias Symbols: RECA, BRCC5, FANCR, MRMV2, HRAD51, RAD51A, HsRad51, HsT16930
• Product Format: Liquid PBS
• Description of Target: Rad51, a 37 kDa protein, is the human homologue of E. coli RecA protein and a member of the RAD52 epistasis group in S. cerevisiae. In recent studies it has been reported that BRCA1 interacts with Rad51 and disease-causing mutations have been found in the BRCA1 region necessary for BRCA1/Rad51 interaction, implying that this interaction is important for tumor suppression. BRCA2 and Rad51 have also been shown to interact by direct binding of the BRC repeats located within exon 11 of BRCA2. When these repeats are deleted, the interaction is lost and cells become hypersensitive to the DNA damage caused by methyl methanesulfonate (MMS).
• Reconstitution and Storage: Keep as concentrated solution. Store at 4°C short term (1-2 weeks). For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
• Additional Information: Positive Controls: Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat-2
• Application Info: WB: 1:500~3000
  ICC/IF: 1:100~1000
  IHC-P: 1:100~1000
  IP: Assay dependent
  PLA: Assay dependent
• Immunogen: Full length (amino acids 1-338) Rad51 expressed in E. coli.
• Uniprot ID: Q06609
• Protein Name: DNA repair protein RAD51 homolog 1
• Protein Accession #: NP_001157741.1
• Purification: Antigen affinity chromatography
• Nucleotide Accession #: NM_001164269.1
• Gene Symbol: RAD51
• Predicted Species Reactivity: Human, Mouse, Rat, Chicken
• Application: ICC, IF, IHC-P, IP, WB
• Image 1: U2OS cells
  
  Immunofluorecent staning of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody .
  Cells were pre-extracted with CSK buffer before fixation with 4% PFA.
  RAD51 14B4 was used at 1:1000 dultion. DAPI was used to counterstain the nucleus.
  Scale bar, 10 mciro-meter.
• Image 2: BT483 xenograft
  
  RAD51 antibody 14B4 detects RAD51 in nucleus on BT483 xenograft by immunohistochemical analysis.
  Sample: Paraffin-embedded BT483 xenograft.
  RAD51 antibody 14B4 dilution: 1:200.
• Image 3: HeLa cell
  
  Mouse anti-RAD51 14B4 antibody was used in IP assay (immunoprecipitation) using HeLa cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40).
  Rabbit anti-RAD51 antibody was used for subsequent WB detection of immunoprecipated RAD51.
  Whole cell extract was used as input.
• Image 4: 293T whole cell
  
  Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
• Image 5: Whole cell
  
  Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.