| Product Number |
OAGA00449 |
| Product Page |
www.avivasysbio.com/dclk2-antibody-oaga00449.html |
| Name |
DCLK2 Antibody (OAGA00449) |
| Molecular Weight |
84 kDa |
| Isotype |
IgG |
| NCBI Gene Id |
166614 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.16 mg/ml |
| Gene Full Name |
Serine/threonine-protein kinase DCLK2 |
| Alias Symbols |
CL2, DCK2, CLIK2, DCDC3, CLICK2, DCDC3B, DCAMKL2, CLICK-II |
| Product Format |
Liquid |
| Reconstitution and Storage |
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-DCLK2 antibody - OAGA00449 |
| Additional Information |
Background: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq] |
| Additional Information |
Positive Controls: 293T, H1299, HepG2, Molt-4, Raji |
| Application Info |
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Immunogen |
Recombinant fragment corresponding to a region within amino acids 187 and 522 of DCAMKL2 (Uniprot ID#Q8N568) |
| Storage Buffer |
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Uniprot ID |
Q8N568 |
| Protein Name |
Serine/threonine-protein kinase DCLK2 |
| Protein Accession # |
NP_001035350.2 |
| Purification |
Purified by antigen-affinity chromatography. |
| Gene Symbol |
DCLK2 |
| Predicted Species Reactivity |
Human |
| Application |
ICC, IF, WB |
| Image 1 | H1299
 | Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
OAGA00449 diluted at 1:500 |
| | Image 2 | HepG2
 | DCAMKL2 antibody [N2C2], Internal detects DCAMKL2 protein at Cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in -20? 100% MeOH for 5 min.
Green: DCAMKL2 protein stained by DCAMKL2 antibody [N2C2], Internal (OAGA00449) diluted at 1:500.
Blue: Hoechst 33343 staining.
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