dnaK Antibody (OAED00201)

• Product Number: OAED00201
• Name: dnaK Antibody (OAED00201)
• Clone: 8E2/2
• Isotype: IgG1
• NCBI Gene Id: 944750
• Host: Mouse
• Clonality: Monoclonal
• Gene Full Name: chaperone protein DnaK
• Description:
  View product citations for dnaK Antibody (OAED00201) on CiteAb
• Alias Symbols: b0014;chaperone protein DnaK;ECK0014;groPAB;groPC;groPF;grpC;grpF;Heat shock 70 kDa protein;Heat shock protein 70;HSP70;seg.
• Product Format: Liquid. PBS (ph 7.2), containing 50% glycerol and 0.09% sodium azide.
• Reference: Scope and limitations of the designer proline-rich antibacterial peptide dimer, A3-APO, alone or in synergy with conventional antibiotics: J.r. Otvos, et al. ; Peptides 29, 1878 (2008), Application(s): WB using bacteria purified protein, Abstract;
  TLR4-mediated activation of dendritic cells by the heat shock protein DnaK from Francisella tularensis: S. Michalek, et al. ; J. Leukoc. Biol. 84, 1434 (2008), Application(s): WB using bacteria cell lysates, Abstract;
  An essential role for the DnaK molecular chaperone in stabilizing over-expressed substrate proteins of the bacterial twin-arginine translocation pathway: M. DeLisa, et al. ; J. Mol. Biol. 367, 715 (2007), Application(s): WB using bacteria cell lysates, Abstract;
  Decline in ribosomal fidelity contributes to the accumulation and stabilization of the master stress response regulator ?(S) upon carbon starvation: T. Nystroem, et al. ; Genes Dev. 21, 862 (2007), Application(s): WB using bacteria cell lysates, Abstract;
  Escherichia coli heat shock protein DnaK: production and consequences in terms of monitoring cooking: L. Saucier, et al. ; Appl. Environ. Microbiol. 69, 3231 (2003), Application(s): EIA using bacteria (E. coli) samples, Abstract;
  Secretin of the enteropathogenic Escherichia coli type III secretion system requires components of the type III apparatus for assembly and localization: B.B. Finlay, et al. ; Infect. Immun. 71, 3310 (2003), Application(s): WB using bacteria (E. coli) samples, Abstract;
  Salmonella effectors within a single pathogenicity island are differentially expressed and translocated by separate type III secretion systems: B.B. Finlay, et al. ; Mol. Microbiol. 43, 1089 (2002), Application(s): WB using bacteria (Salmonella) samples, Abstract;
  Adaptive and cross-protective responses of Pseudomonas sp. DJ-12 to several aromatics and other stress shocks: C.K. Kim, et al. ; Curr. Microbiol. 43, 176 (2001), Application(s): WB using bacteria (porcineseudomonas) samples, Abstract;
  Detection of stress proteins in Porphyromonas gingivalis and other oral bacteria by Western immunoblotting analysis: D. Grenier, et al. ; FEMS Microbiol. Lett. 121, 303 (1994), Application(s): WB using bacteria (Actinomyces, Capnocytophaga sp.) samples, Abstract;
  
• Description of Target: Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the DnaA protein. Also participates actively in the response to hyperosmotic shock.
• Reconstitution and Storage: Store long term at -20C.
• Specificity: Recognizes bacteria DnaK. Detects a band of ~70kDa by Western blot.
• Application Info: WB 1:10000
• Immunogen: E. coli DnaK.
• Uniprot ID: P0A6Y8
• Protein Name: Chaperone protein DnaK
• Publications:

  Aggregating sequences that occur in many proteins constitute weak spots of bacterial proteostasis. Nat Commun. 9, 866 (2018). 29491361

• Purification: Protein G affinity purified.
• Gene Symbol: dnaK
• Predicted Species Reactivity: Escherichia coli
• Application: Western blot
• Image 1: DnaK active recombinant protein, E. coli cell lysate
  
  Western blot analysis: Lane 1: MW Marker; Lane 2: DnaK active recombinant protein; Lane 3: E. coli cell lysate.