dnaK Antibody (OAED00201)

Catalog No: OAED00201

Size:50 ug

Western blot analysis: Lane 1: MW Marker; Lane 2: DnaK active recombinant protein; Lane 3: E. coli cell lysate.

Product Info

• Predicted Species Reactivity: Bacteria
• Product Format: Liquid. PBS (ph 7.2), containing 50% glycerol and 0.09% sodium azide.
• Clonality: Monoclonal
• Clone: 8E2/2
• Isotype: IgG1
• Host: Mouse
• Application: WB
• Reconstitution and Storage: Store long term at -20C.
• Immunogen: E. coli DnaK.
• Purification: Protein G affinity purified.
• Specificity: Recognizes bacteria DnaK. Detects a band of ~70kDa by Western blot.
• Application Info: WB 1:10000
• Publications:

  Aggregating sequences that occur in many proteins constitute weak spots of bacterial proteostasis. Nat Commun. 9, 866 (2018). 29491361

• Reference: Scope and limitations of the designer proline-rich antibacterial peptide dimer, A3-APO, alone or in synergy with conventional antibiotics: J.r. Otvos, et al. ; Peptides 29, 1878 (2008), Application(s): WB using bacteria purified protein, Abstract;
  TLR4-mediated activation of dendritic cells by the heat shock protein DnaK from Francisella tularensis: S. Michalek, et al. ; J. Leukoc. Biol. 84, 1434 (2008), Application(s): WB using bacteria cell lysates, Abstract;
  An essential role for the DnaK molecular chaperone in stabilizing over-expressed substrate proteins of the bacterial twin-arginine translocation pathway: M. DeLisa, et al. ; J. Mol. Biol. 367, 715 (2007), Application(s): WB using bacteria cell lysates, Abstract;
  Decline in ribosomal fidelity contributes to the accumulation and stabilization of the master stress response regulator σ(S) upon carbon starvation: T. Nystroem, et al. ; Genes Dev. 21, 862 (2007), Application(s): WB using bacteria cell lysates, Abstract;
  Escherichia coli heat shock protein DnaK: production and consequences in terms of monitoring cooking: L. Saucier, et al. ; Appl. Environ. Microbiol. 69, 3231 (2003), Application(s): EIA using bacteria (E. coli) samples, Abstract;
  Secretin of the enteropathogenic Escherichia coli type III secretion system requires components of the type III apparatus for assembly and localization: B.B. Finlay, et al. ; Infect. Immun. 71, 3310 (2003), Application(s): WB using bacteria (E. coli) samples, Abstract;
  Salmonella effectors within a single pathogenicity island are differentially expressed and translocated by separate type III secretion systems: B.B. Finlay, et al. ; Mol. Microbiol. 43, 1089 (2002), Application(s): WB using bacteria (Salmonella) samples, Abstract;
  Adaptive and cross-protective responses of Pseudomonas sp. DJ-12 to several aromatics and other stress shocks: C.K. Kim, et al. ; Curr. Microbiol. 43, 176 (2001), Application(s): WB using bacteria (porcineseudomonas) samples, Abstract;
  Detection of stress proteins in Porphyromonas gingivalis and other oral bacteria by Western immunoblotting analysis: D. Grenier, et al. ; FEMS Microbiol. Lett. 121, 303 (1994), Application(s): WB using bacteria (Actinomyces, Capnocytophaga sp.) samples, Abstract;
  

Target Info

• Gene Symbol: DnaK
• NCBI Gene Id: AP009048
• Protein Name: Chaperone protein DnaK
• Description of Target: DnaK possesses an autophosphorylation activity and a weak 5-nucleotidase activity, cleaving the 5 phosphate groups from both ribose and deoxyribose nucleotides. The role of DnaK in ATP-dependent protein-protein interactions has been extended to normal E. coli physiology, where, like eukaryotic Hsp70 homologs, it is thought to participate in the assembly/disassembly of protein complexes.
• Uniprot ID: P0A6Y8 (strain K12)