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Catalog No: OAED00201
Size:50 ug
Price: $269.00
SKU
OAED00201
Availability: Domestic: within 1-2 week delivery | International: 1-2 week
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Datasheets/ManualsPrintable datasheet for dnaK Antibody (OAED00201) (OAED00201)
Product Info
Predicted Species ReactivityEscherichia coli
Product FormatLiquid. PBS (ph 7.2), containing 50% glycerol and 0.09% sodium azide.
ClonalityMonoclonal
Clone8E2/2
IsotypeIgG1
HostMouse
ApplicationWestern blot
Reconstitution and StorageStore long term at -20C.
ImmunogenE. coli DnaK.
PurificationProtein G affinity purified.
SpecificityRecognizes bacteria DnaK. Detects a band of ~70kDa by Western blot.
Application InfoWB 1:10000
Publications

Aggregating sequences that occur in many proteins constitute weak spots of bacterial proteostasis. Nat Commun. 9, 866 (2018). 29491361

ReferenceScope and limitations of the designer proline-rich antibacterial peptide dimer, A3-APO, alone or in synergy with conventional antibiotics: J.r. Otvos, et al. ; Peptides 29, 1878 (2008), Application(s): WB using bacteria purified protein, Abstract;
TLR4-mediated activation of dendritic cells by the heat shock protein DnaK from Francisella tularensis: S. Michalek, et al. ; J. Leukoc. Biol. 84, 1434 (2008), Application(s): WB using bacteria cell lysates, Abstract;
An essential role for the DnaK molecular chaperone in stabilizing over-expressed substrate proteins of the bacterial twin-arginine translocation pathway: M. DeLisa, et al. ; J. Mol. Biol. 367, 715 (2007), Application(s): WB using bacteria cell lysates, Abstract;
Decline in ribosomal fidelity contributes to the accumulation and stabilization of the master stress response regulator σ(S) upon carbon starvation: T. Nystroem, et al. ; Genes Dev. 21, 862 (2007), Application(s): WB using bacteria cell lysates, Abstract;
Escherichia coli heat shock protein DnaK: production and consequences in terms of monitoring cooking: L. Saucier, et al. ; Appl. Environ. Microbiol. 69, 3231 (2003), Application(s): EIA using bacteria (E. coli) samples, Abstract;
Secretin of the enteropathogenic Escherichia coli type III secretion system requires components of the type III apparatus for assembly and localization: B.B. Finlay, et al. ; Infect. Immun. 71, 3310 (2003), Application(s): WB using bacteria (E. coli) samples, Abstract;
Salmonella effectors within a single pathogenicity island are differentially expressed and translocated by separate type III secretion systems: B.B. Finlay, et al. ; Mol. Microbiol. 43, 1089 (2002), Application(s): WB using bacteria (Salmonella) samples, Abstract;
Adaptive and cross-protective responses of Pseudomonas sp. DJ-12 to several aromatics and other stress shocks: C.K. Kim, et al. ; Curr. Microbiol. 43, 176 (2001), Application(s): WB using bacteria (porcineseudomonas) samples, Abstract;
Detection of stress proteins in Porphyromonas gingivalis and other oral bacteria by Western immunoblotting analysis: D. Grenier, et al. ; FEMS Microbiol. Lett. 121, 303 (1994), Application(s): WB using bacteria (Actinomyces, Capnocytophaga sp.) samples, Abstract;
Description
Gene SymboldnaK
Gene Full Namechaperone protein DnaK
Alias Symbolsb0014;chaperone protein DnaK;ECK0014;groPAB;groPC;groPF;grpC;grpF;Heat shock 70 kDa protein;Heat shock protein 70;HSP70;seg.
NCBI Gene Id944750
Protein NameChaperone protein DnaK
Description of TargetPlays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the DnaA protein. Also participates actively in the response to hyperosmotic shock.
Uniprot IDP0A6Y8
  1. What is the species homology for "dnaK Antibody (OAED00201)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Escherichia coli".

  2. How long will it take to receive "dnaK Antibody (OAED00201)"?

    This item is available "Domestic: within 1-2 week delivery | International: 1-2 week".

  3. What buffer format is "dnaK Antibody (OAED00201)" provided in?

    This item is provided in "Liquid. PBS (ph 7.2), containing 50% glycerol and 0.09% sodium azide.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "dnaK Antibody (OAED00201)"?

    This target may also be called "b0014;chaperone protein DnaK;ECK0014;groPAB;groPC;groPF;grpC;grpF;Heat shock 70 kDa protein;Heat shock protein 70;HSP70;seg." in publications.

  5. What is the shipping cost for "dnaK Antibody (OAED00201)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "dnaK Antibody (OAED00201)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "dnaK Antibody (OAED00201)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "dnaK Antibody (OAED00201)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "dnaK"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "dnaK"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "dnaK"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "dnaK"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "dnaK"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "dnaK"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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