| Product Number |
OAEB02354 |
| Product Page |
www.avivasysbio.com/cd14-antibody-oaeb02354.html |
| Name |
CD14 Antibody (OAEB02354) |
| Molecular Weight |
40.1 |
| NCBI Gene Id |
929 |
| Host |
Goat |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
CD14 molecule |
| Alias Symbols |
monocyte differentiation antigen CD14;myeloid cell-specific leucine-rich glycoprotein. |
| Peptide Sequence |
C-KRVDADADPRQYAD |
| Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reference |
* Iwaki D, Nishitani C, Mitsuzawa H, Hyakushima N, Sano H, Kuroki Y.; The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain.; Biochem Biophys Res Commun. 2005 Mar 4;328(1):173-6.; Pubmed 15670766 |
| Reconstitution and Storage |
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Datasheets/Manuals |
Printable datasheet for CD14 Antibody (OAEB02354) |
| Application Info |
Peptide ELISA: antibody detection limit dilution 1:128000.
Western Blot: Approx 55 kDa band observed in Human Peripheral Blood Mononucleocytes (PBM) lysates (calculated MW of 40.1kDa according to NP_000582.1).The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 15 kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation is 1 hour at room temperature. |
| Immunogen |
CD14 |
| Uniprot ID |
P08571 |
| Protein Name |
Monocyte differentiation antigen CD14 |
| Publications |
Human annulus progenitor cells: Analyses of this viable endogenous cell population. J. Orthop. Res. 34, 1351-60 (2016). 27249627 |
| Protein Accession # |
NP_000582.1 |
| Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
| Tested Species Reactivity |
Human |
| Gene Symbol |
CD14 |
| Predicted Species Reactivity |
Human |
| Application |
WB |
| Image 1 | Human Lymph Node
 | OAEB02354 (1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
| | Image 2 | Human Spleen
 | OAEB02354(4ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
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