Product Number |
OAEB02329 |
Product Page |
www.avivasysbio.com/goat-anti-crhr1-crf-r-aa-107-117-antibody-oaeb02329.html |
Name |
Goat Anti-CRHR1 / CRF-R (aa 107 - 117) Antibody (OAEB02329) |
Molecular Weight |
47.7 |
NCBI Gene Id |
12921|1394|58959 |
Host |
Goat |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
corticotropin releasing hormone receptor 1 |
Description |
|
Alias Symbols |
corticotrophin releasing hormone receptor 1;corticotropin releasing hormone 1;corticotropin-releasing factor receptor 1;corticotropin-releasing factor type 1 receptor;CRF;CRF 1 receptor;CRF1;CRF1R;CRF-R;CRFR1;CRFR-1;CRF-R1;CRF-R-1;CRF-R1alpha;Crhr;CRH-R 1;CRH-R1;CRH-R-1;CRHR1L;seven transmembrane helix receptor. |
Peptide Sequence |
C-NEEKKSKVHYH |
Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Reference |
* Rissman RA, Lee KF, Vale W, Sawchenko PE.; Corticotropin-releasing factor receptors differentially regulate stress-induced tau phosphorylation.; J Neurosci. 2007 Jun 13;27(24):6552-62.; Pubmed 17567816 |
Reconstitution and Storage |
Aliquot and store at -20°C. Minimize freezing and thawing. |
Datasheets/Manuals |
Printable datasheet for Goat Anti-CRHR1 / CRF-R (aa 107 - 117) Antibody (OAEB02329) |
Application Info |
P-ELISA: Antibody detection limit dilution 1:128000 WB: Approx 60 kDa band observed in Human Brain (Cerebellum), Colon, Rat Brain and Mouse spinal cord lysates (calculated MW of 47.7 kDa according to NP_004373.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu et al, Mol Pharmacol. 2001 Mar;59(3):485-92; PMID: 11179443) . Recommended concentration: 0.1-0.3 ug/ml. Primary incubation was 1 hour. |
Other Applications Image 1 Data |
Confocal image (3um) of OAEB02329 (0.3ug/ml) staining in green a dendrite of a neuronal cell in C57 mouse cortex. Counter-stained with DAPI (in blue). Data kindly provided by Dr Y. Chen, TZ Baram lab, UC Irvine, CA. |
Immunogen |
CRHR1 |
Uniprot ID |
P34998 |
Protein Name |
Corticotropin-releasing factor receptor 1 |
Publications |
Control of locomotor speed, arousal, and hippocampal theta rhythms by the nucleus incertus. Nat Commun. 11, 262 (2020). 31937768 |
Protein Accession # |
NP_004373.2 |
Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
Application Data |
This antibody has been successfully used in ICC/IF on Rat: Zhong Xie, Peter Penzes, Deepak P. Srivastava EPAC2 is required for corticotropin-releasing hormone-mediated spine loss April 2019: doi: https://doi.org/10.1101/607598 PMID: 0 This antibody has been successfully used in Western blot on Mouse: Baglietto-Vargas D, Chen Y, Suh D, Ager RR, Rodriguez-Ortiz CJ, Medeiros R, Myczek K, Green KN, Baram TZ, LaFerla FM.
Short-term modern life-like stress exacerbates A-beta-pathology and synapse loss in 3xTg-AD mice. J Neurochem. 2015 Sep;134(5):915-26. PMID: 26077803 This antibody has been successfully used in Western blot on Mouse: Weerachayaphorn J, Mennone A, Soroka CJ, Harry K, Hagey LR, Kensler TW, Boyer JL. Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine. Am J Physiol Gastrointest Liver Physiol. 2012 May 1;302(9):G925-36. PMID: 22345550 This antibody has been successfully used in IHC on Mouse: Jungling K, Liu X, Lesting J, Coulon P, Sosulina L, Reinscheid RK, Pape HC. Activation of neuropeptide S expressing neurons in the locus coeruleus by corticotropin-releasing factor. J Physiol. 2012 May 8. PMID: 22570383. |
Tested Species Reactivity |
Human, Mouse, Rat |
Gene Symbol |
Crhr1|CRHR1 |
Predicted Species Reactivity |
Human|Mouse|Rat |
Application |
WB, P-ICC, IF |
Image 1 | human colon
| OAEB02329 (0.1ug/ml) staining of human colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
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Image 2 | mouse cortex
| Confocal image (3um) of OAEB02329 (0.3ug/ml) staining in green a dendrite of a neuronal cell in C57 mouse cortex. Counter-stained with DAPI (in blue). Data kindly provided by Dr Y. Chen, TZ Baram lab, UC Irvine, CA. |
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