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Goat Anti-CRHR1 / CRF-R (aa 107 - 117) Antibody (OAEB02329)
Datasheets/Manuals | Printable datasheet for Goat Anti-CRHR1 / CRF-R (aa 107 - 117) Antibody (OAEB02329) |
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Tested Species Reactivity | Human, Mouse, Rat |
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Predicted Species Reactivity | Human|Mouse|Rat |
Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | WB, P-ICC, IF |
Reconstitution and Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Immunogen | CRHR1 |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Peptide Sequence | C-NEEKKSKVHYH |
Concentration | 0.5 mg/ml |
Application Info | P-ELISA: Antibody detection limit dilution 1:128000 WB: Approx 60 kDa band observed in Human Brain (Cerebellum), Colon, Rat Brain and Mouse spinal cord lysates (calculated MW of 47.7 kDa according to NP_004373.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu et al, Mol Pharmacol. 2001 Mar;59(3):485-92; PMID: 11179443) . Recommended concentration: 0.1-0.3 ug/ml. Primary incubation was 1 hour. |
Application Data | This antibody has been successfully used in ICC/IF on Rat: Zhong Xie, Peter Penzes, Deepak P. Srivastava EPAC2 is required for corticotropin-releasing hormone-mediated spine loss April 2019: doi: https://doi.org/10.1101/607598 PMID: 0 This antibody has been successfully used in Western blot on Mouse: Baglietto-Vargas D, Chen Y, Suh D, Ager RR, Rodriguez-Ortiz CJ, Medeiros R, Myczek K, Green KN, Baram TZ, LaFerla FM. Short-term modern life-like stress exacerbates A-beta-pathology and synapse loss in 3xTg-AD mice. J Neurochem. 2015 Sep;134(5):915-26. PMID: 26077803 This antibody has been successfully used in Western blot on Mouse: Weerachayaphorn J, Mennone A, Soroka CJ, Harry K, Hagey LR, Kensler TW, Boyer JL. Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine. Am J Physiol Gastrointest Liver Physiol. 2012 May 1;302(9):G925-36. PMID: 22345550 This antibody has been successfully used in IHC on Mouse: Jungling K, Liu X, Lesting J, Coulon P, Sosulina L, Reinscheid RK, Pape HC. Activation of neuropeptide S expressing neurons in the locus coeruleus by corticotropin-releasing factor. J Physiol. 2012 May 8. PMID: 22570383. |
Other Applications Image 1 Data | Confocal image (3um) of OAEB02329 (0.3ug/ml) staining in green a dendrite of a neuronal cell in C57 mouse cortex. Counter-stained with DAPI (in blue). Data kindly provided by Dr Y. Chen, TZ Baram lab, UC Irvine, CA. |
Reference | * Rissman RA, Lee KF, Vale W, Sawchenko PE.; Corticotropin-releasing factor receptors differentially regulate stress-induced tau phosphorylation.; J Neurosci. 2007 Jun 13;27(24):6552-62.; Pubmed 17567816 |
Publications | Control of locomotor speed, arousal, and hippocampal theta rhythms by the nucleus incertus. Nat Commun. 11, 262 (2020). 31937768 |
Description |
Gene Symbol | Crhr1|CRHR1 |
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Gene Full Name | corticotropin releasing hormone receptor 1 |
Alias Symbols | corticotrophin releasing hormone receptor 1;corticotropin releasing hormone 1;corticotropin-releasing factor receptor 1;corticotropin-releasing factor type 1 receptor;CRF;CRF 1 receptor;CRF1;CRF1R;CRF-R;CRFR1;CRFR-1;CRF-R1;CRF-R-1;CRF-R1alpha;Crhr;CRH-R 1;CRH-R1;CRH-R-1;CRHR1L;seven transmembrane helix receptor. |
NCBI Gene Id | 12921|1394|58959 |
Protein Name | Corticotropin-releasing factor receptor 1 |
Uniprot ID | P34998 |
Protein Accession # | NP_004373.2 |
Molecular Weight | 47.7 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Goat Anti-CRHR1 / CRF-R (aa 107 - 117) Antibody (OAEB02329)"?
The tested species reactivity for this item is "Human, Mouse, Rat". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "Goat Anti-CRHR1 / CRF-R (aa 107 - 117) Antibody (OAEB02329)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Goat Anti-CRHR1 / CRF-R (aa 107 - 117) Antibody (OAEB02329)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Goat Anti-CRHR1 / CRF-R (aa 107 - 117) Antibody (OAEB02329)"?
This target may also be called "corticotrophin releasing hormone receptor 1;corticotropin releasing hormone 1;corticotropin-releasing factor receptor 1;corticotropin-releasing factor type 1 receptor;CRF;CRF 1 receptor;CRF1;CRF1R;CRF-R;CRFR1;CRFR-1;CRF-R1;CRF-R-1;CRF-R1alpha;Crhr;CRH-R 1;CRH-R1;CRH-R-1;CRHR1L;seven transmembrane helix receptor." in publications.
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What is the shipping cost for "Goat Anti-CRHR1 / CRF-R (aa 107 - 117) Antibody (OAEB02329)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Goat Anti-CRHR1 / CRF-R (aa 107 - 117) Antibody (OAEB02329)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Goat Anti-CRHR1 / CRF-R (aa 107 - 117) Antibody (OAEB02329)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "47.7".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Goat Anti-CRHR1 / CRF-R (aa 107 - 117) Antibody (OAEB02329)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Crhr1|CRHR1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Crhr1|CRHR1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Crhr1|CRHR1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Crhr1|CRHR1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Crhr1|CRHR1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Crhr1|CRHR1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.