Goat Anti-CRHR1 / CRF-R (aa 107 - 117) Antibody (OAEB02329)

Catalog No: OAEB02329 (Formerly GWB-EV0117)

Size:100ug

OAEB02329 (0.1ug/ml) staining of human colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Confocal image (3um) of OAEB02329 (0.3ug/ml) staining in green a dendrite of a neuronal cell in C57 mouse cortex. Counter-stained with DAPI (in blue). Data kindly provided by Dr Y. Chen, TZ Baram lab, UC Irvine, CA.

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, P-ICC, IF
• Reconstitution and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Immunogen: Peptide with sequence C-NEEKKSKVHYH, from the internal region of the protein sequence according to NP_004373.2.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Concentration: 0.5 mg/ml
• Application Info: P-ELISA: Antibody detection limit dilution 1:128000
  WB: Approx 60 kDa band observed in Human Brain (Cerebellum), Colon, Rat Brain and Mouse spinal cord lysates (calculated MW of 47.7 kDa according to NP_004373.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu et al, Mol Pharmacol. 2001 Mar;59(3):485-92; PMID: 11179443) . Recommended concentration: 0.1-0.3 ug/ml. Primary incubation was 1 hour.
• Application Data: This antibody has been successfully used in ICC/IF on Rat:
  Zhong Xie, Peter Penzes, Deepak P. Srivastava
  EPAC2 is required for corticotropin-releasing hormone-mediated spine loss
  April 2019: doi: https://doi.org/10.1101/607598
  PMID: 0
  This antibody has been successfully used in Western blot on Mouse:
  Baglietto-Vargas D, Chen Y, Suh D, Ager RR, Rodriguez-Ortiz CJ, Medeiros R, Myczek K, Green KN, Baram TZ, LaFerla FM.
  Short-term modern life-like stress exacerbates A-beta-pathology and synapse loss in 3xTg-AD mice.
  J Neurochem. 2015 Sep;134(5):915-26.
  PMID: 26077803
  This antibody has been successfully used in Western blot on Mouse:
  Weerachayaphorn J, Mennone A, Soroka CJ, Harry K, Hagey LR, Kensler TW, Boyer JL.
  Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine.
  Am J Physiol Gastrointest Liver Physiol. 2012 May 1;302(9):G925-36.
  PMID: 22345550
  This antibody has been successfully used in IHC on Mouse:
  Jungling K, Liu X, Lesting J, Coulon P, Sosulina L, Reinscheid RK, Pape HC.
  Activation of neuropeptide S expressing neurons in the locus coeruleus by corticotropin-releasing factor.
  J Physiol. 2012 May 8.
  PMID: 22570383.
• Other Applications Image 1 Data: Confocal image (3um) of OAEB02329 (0.3ug/ml) staining in green a dendrite of a neuronal cell in C57 mouse cortex. Counter-stained with DAPI (in blue). Data kindly provided by Dr Y. Chen, TZ Baram lab, UC Irvine, CA.
• Reference: * Rissman RA, Lee KF, Vale W, Sawchenko PE.; Corticotropin-releasing factor receptors differentially regulate stress-induced tau phosphorylation.; J Neurosci. 2007 Jun 13;27(24):6552-62.; Pubmed 17567816
• Publications:

  Control of locomotor speed, arousal, and hippocampal theta rhythms by the nucleus incertus. Nat Commun. 11, 262 (2020). 31937768

Target Info

• Gene Symbol: CRHR1
• Alias Symbols: CRF1, CRHR, CRF-R, CRFR1, CRF-R1, CRFR-1, CRH-R1, CRHR1L, CRF-R-1, CRH-R-1
• NCBI Gene Id: 1394
• Protein Name: Corticotropin-releasing factor receptor 1
• Uniprot ID: P34998
• Protein Accession #: NP_004373.2