HDAC3 Antibody (OADC00269)

• Product Number: OADC00269
• Name: HDAC3 Antibody (OADC00269)
• Isotype: IgG1
• Conjugation: Unconjugated
• NCBI Gene Id: 8841;
• Host: Mouse
• Clonality: Monoclonal
• Concentration: 2 mg/mL
• Gene Full Name: histone deacetylase 3
• Alias Symbols: HD3, RPD3, KDAC3, RPD3-2
• Product Format: Liquid. Provided in PBS containing 0.05% azide and 0.05% ProClin 300.
• Description of Target: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
• Reconstitution and Storage: Store at -20C; for long storage, store at -80C. Avoid repeated freeze/thaw cycles.
• Specificity: Mouse monoclonal antibody raised against HDAC3
• Application Info: ChIP: 2 ug/ChIP
  IF: 1:500
• Immunogen: Monoclonal antibody raised in mouse against human HDAC3 (Histone deacetylase 3), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal region of the protein.
• Uniprot ID: O15379
• Protein Name: histone deacetylase 3
• Protein Accession #: NP_003874.2
• Purification: Protein A/G purified
• Nucleotide Accession #: NM_003883.3
• Gene Symbol: HDAC3
• Predicted Species Reactivity: Human
• Application: CHIP, IF
• Image 1: Human Hela cells
  
  ChIP results obtained with the Aviva monoclonal antibody directed against HDAC3. ChIP assays were performed using human HeLa cells, the Aviva monoclonal antibody against HDAC3 and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 10,000 cells using the SX-8G IP-Star automated system. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and GAPDH, and for the coding region of p21, a known target gene of HDAC3. Figure 4 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
• Image 2: Hela cells
  
  Immunofluorescence using the Aviva monoclonal antibody directed against HDAC3r. HeLa cells were stained with the Aviva antibody against HDAC3 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC3 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.