| Product Number |
OACD05972 |
| Product Page |
www.avivasysbio.com/lcn2-antibody-oacd05972.html |
| Name |
LCN2 Antibody (OACD05972) |
| Molecular Weight |
23 kDa |
| Isotype |
IgG |
| NCBI Gene Id |
16819 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1 mg/ml |
| Gene Full Name |
lipocalin 2 |
| Alias Symbols |
24p;24p3;AW212229;Lipocalin-2;N;neu-related lipocalin;neutrophil gelatinase-associated lipocalin;NRL;oncogene 24p3;p25;secreted inducible protein 24;siderocalin LCN2;Sip24;SV-40-induced 24P3 protein. |
| Product Format |
Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. |
| Description of Target |
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. |
| Reconstitution and Storage |
2°C to 8°C|-20°C |
| Datasheets/Manuals |
Printable datasheet for LCN2 Antibody (OACD05972) |
| Specificity |
The antibody is a rabbit polyclonal antibody raised against NGAL. It has been selected for its ability to recognize NGAL in immunohistochemical staining and western blotting. |
| Immunogen |
Range: Gln21 - Asn200 |
| Formulation |
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol |
| Uniprot ID |
P11672 |
| Protein Name |
Neutrophil gelatinase-associated lipocalin |
| Protein Accession # |
NP_032517.1 |
| Purification |
Affinity Purified; Protein A Purified |
| Application Data |
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-1:5,000
Optimal working dilutions must be determined by end user. |
| Nucleotide Accession # |
NM_008491 |
| Preservative |
0.02% NaN3 |
| Gene Symbol |
Lcn2 |
| Predicted Species Reactivity |
Mouse |
| Application |
Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot |
| Image 1 | Neutrophil gelatinase-associated lipocalin
 | Western Blot with Mouse Recombinant Neutrophil gelatinase-associated lipocalin |
| | Image 2 | Lung tissue
 | DAB staining on formalin fixed paraffin-embedded Lung tissue |
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