LCN2 Antibody (OACD05972)

Catalog No: OACD05972

Western Blot with Mouse Recombinant Neutrophil gelatinase-associated lipocalin

DAB staining on formalin fixed paraffin-embedded Lung tissue

Western Blot with Mouse Recombinant Neutrophil gelatinase-associated lipocalin

DAB staining on formalin fixed paraffin-embedded Lung tissue

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Range: Gln21 - Asn200
• Purification: Affinity Purified; Protein A Purified
• Concentration: 1 mg/ml
• Preservative: 0.02% NaN3
• Specificity: The antibody is a rabbit polyclonal antibody raised against NGAL. It has been selected for its ability to recognize NGAL in immunohistochemical staining and western blotting.
• Formulation: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol
• Application Data: Western blotting: 1:50-400
  Immunocytochemistry in formalin fixed cells: 1:50-500
  Immunohistochemistry in formalin fixed frozen section: 1:50-500
  Immunohistochemistry in paraffin section: 1:10-100
  Enzyme-linked Immunosorbent Assay: 1:100-1:5,000
  Optimal working dilutions must be determined by end user.

Target Info

• Gene Symbol: Lcn2
• Gene Full Name: lipocalin 2
• Alias Symbols: 24p;24p3;AW212229;Lipocalin-2;N;neu-related lipocalin;neutrophil gelatinase-associated lipocalin;NRL;oncogene 24p3;p25;secreted inducible protein 24;siderocalin LCN2;Sip24;SV-40-induced 24P3 protein.
• NCBI Gene Id: 16819
• Protein Name: Neutrophil gelatinase-associated lipocalin
• Description of Target: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
• Uniprot ID: P11672
• Protein Accession #: NP_032517.1
• Nucleotide Accession #: NM_008491
• Molecular Weight: 23 kDa