NFKB1 Antibody (OACA02427)

• Product Number: OACA02427
• Name: NFKB1 Antibody (OACA02427)
• Protein Size (# AA): 968 amino acids
• Molecular Weight: 105 kDa
• Isotype: IgG
• Conjugation: Unconjugated
• NCBI Gene Id: 4790
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: nuclear factor kappa B subunit 1
• Alias Symbols: CVID12;DNA-binding factor KBF1;EBP-1;KBF1;NFkappaB;NF-kappaB;NF-kappa-B1;NF-kappabeta;NF-kB;NF-kB1;NFKB-p105;NFKB-p50;nuclear factor kappa-B DNA binding subunit;nuclear factor NF-kappa-B p105 subunit;nuclear factor NF-kappa-B p50 subunit;nuclear factor of kappa light polypeptide gene enhancer in B-cells 1.
• Product Format: Liquid
• Reference: [1] The DNA binding subunit of NF-kappa B is identical to factor KBF1 and homologous to the rel oncogene product. Kieran M., Blank V., Logeat F., Vandekerckhove J., Lottspeich F., le Bail O., Urban M.B., Kourilsky P., Baeuerle P.A., Israel A.Cell 62:1007.
• Description of Target: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.
• Reconstitution and Storage: -20°C or -80°C
• Specificity: Human
• Immunogen: Recombinant Human Nuclear factor NF-kappa-B p105 subunit protein (1-961AA)
• Formulation: Provided in PBS, pH 7.4, 50% Glycerol, 0.03% Proclin 300
• Uniprot ID: P19838
• Protein Name: Nuclear factor NF-kappa-B p105 subunit
• Protein Accession #: NP_001158884.1
• Purification: Protein G Purified
• Nucleotide Accession #: NM_001165412.1
• Gene Symbol: NFKB1
• Predicted Species Reactivity: Human
• Application: Chromatin immunoprecipitation|Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Western blot
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: HeLa
  
  Hela (1.1*10e6) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 ug anti-NFKB1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the IkB promoter.
• Image 2: Human Placenta
  
  Immunohistochemistry of paraffin-embedded human placenta tissue at dilution 1: 100
• Image 3: Multiple samples
  
  All lanes: Nuclear factor NF-kappa-B p105 subunit 1 antibody at 2 ug/ml Lane 1: HepG2 whole cell lysate Lane 2: Raji whole cell lysate Goat polyclonal to Rabbit IgG at 1/10000 dilution Predicted band size: 105 kDa Observed band size: 105 kDa