MER/SKY Antibody (Phospho-Tyr749/681) (OAAF00456)

• Product Number: OAAF00456
• Name: MER/SKY Antibody (Phospho-Tyr749/681) (OAAF00456)
• Molecular Weight: 110 kDa
• Isotype: IgG
• NCBI Gene Id: 10461|7301
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: MER proto-oncogene, tyrosine kinase|TYRO3 protein tyrosine kinase
• Alias Symbols: BYK;c-Eyk;c-mer;c-mer proto-oncogene tyrosine kinase;Dtk;Etk-2;MER;MER receptor tyrosine kinase;proto-oncogene c-Mer;receptor tyrosine kinase MerTK;Rek;RP38;RSE;Sky;STK kinase;Tif;Tyro12;tyrosine-protein kinase byk;tyrosine-protein kinase DTK;tyrosine-protein kinase Mer;tyrosine-protein kinase receptor TYRO3;tyrosine-protein kinase RSE;tyrosine-protein kinase SKY;tyrosine-protein kinase TIF.
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Description of Target: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.|Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
• Reconstitution and Storage: -20°C
• Specificity: MER/SKY (Phospho-Tyr749/681) antibody detects endogenous levels of MER/SKY only when phosphorylated at tyrosine 749/681.
• Additional Information: Modification Sites: H: Y749/681, M: Y744/671, R: Y744:671
• Application Info: WB: 1:500~1000
  IHC: 1:50~100
  ELISA: 1:10000
  Optimal dilutions should be determined by the end user.
• Immunogen: The antiserum was produced against synthesized peptide derived from human MER/SKY around the phosphorylation site of Tyr749/681.
• Intended Use: The product is for research use only, not for use in diagnostic or therapeutic procedures.
• Uniprot ID: Q06418|Q12866
• Protein Name: Tyrosine-protein kinase Mer|Tyrosine-protein kinase receptor TYRO3
• Publications:

  Normalization of TAM post-receptor signaling reveals a cell invasive signature for Axl tyrosine kinase. Cell Commun. Signal. 14, 19 (2016). 27595981

• Protein Accession #: NP_006284.2
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Target Post-Translational Modification: Phospho
• Gene Symbol: MERTK|TYRO3
• Predicted Species Reactivity: Human|Mouse|Rat
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Image 1: NIH-3T3 cells
  
  Western blot analysis of lysates from NIH-3T3 cells treated with EGF 200ng/ml 5', using MER/SKY (Phospho-Tyr749/681) Antibody. The lane on the right is blocked with the phospho peptide.
• Image 2: human brain
  
  Immunohistochemistry analysis of paraffin-embedded human brain, using MER/SKY (Phospho-Tyr749/681) Antibody. The picture on the right is blocked with the phospho peptide.