GAK Antibody - C-terminal region (OAAB17420)

• Product Number: OAAB17420
• Name: GAK Antibody - C-terminal region (OAAB17420)
• Molecular Weight: 143191 Da
• Isotype: Ig
• NCBI Gene Id: 2580
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: DNAJ26, DNAJC26
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: GAK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This GAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1140-1170 amino acids from the C-terminal region of human GAK.
• Additional Information: Cellular Location: Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network. Cell junction, focal adhesion (Probable). Note=Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions
  Tissue Location: Ubiquitous. Highest in testis.
• Uniprot ID: O14976
• Protein Name: Cyclin-G-associated kinase
• Sample Type Confirmation:

  GAK is supported by BioGPS gene expression data to be expressed in HEK293

• Protein Accession #: NP_005246.2
• Gene Symbol: GAK
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: 293
  
  Western blot analysis of GAK (arrow) using GAK Antibody (C-term) (Cat.# OAAB17420). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GAK gene (Lane 2) (Origene Technologies).

  GAK is supported by BioGPS gene expression data to be expressed in HEK293

• Image 2: 293
  
  GAK Antibody (V1155) (Cat. # OAAB17420) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the GAK antibody detected the GAK protein (arrow).
• Image 3: Mouse Brain
  
  GAK Antibody (V1155) (Cat. # OAAB17420) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the GAK antibody detected the GAK protein (arrow).
• Image 4: hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.