GAK Antibody - C-terminal region (OAAB17420)

Catalog No: OAAB17420

Size:400ul

Western blot analysis of GAK (arrow) using GAK Antibody (C-term) (Cat.# OAAB17420). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GAK gene (Lane 2) (Origene Technologies).

GAK is supported by BioGPS gene expression data to be expressed in HEK293

GAK Antibody (V1155) (Cat. # OAAB17420) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the GAK antibody detected the GAK protein (arrow).

GAK Antibody (V1155) (Cat. # OAAB17420) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the GAK antibody detected the GAK protein (arrow).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: GAK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1
  Cellular Location: Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network. Cell junction, focal adhesion (Probable). Note=Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions
  Tissue Location: Ubiquitous. Highest in testis.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This GAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1140-1170 amino acids from the C-terminal region of human GAK.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  GAK is supported by BioGPS gene expression data to be expressed in HEK293

• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: GAK
• Alias Symbols: DNAJ26, DNAJC26
• NCBI Gene Id: 2580
• Protein Name: Cyclin-G-associated kinase
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: O14976
• Protein Accession #: NP_005246.2
• Molecular Weight: 143191 Da