BRD2 Antibody - middle region (OAAB17408)

• Product Number: OAAB17408
• Name: BRD2 Antibody - middle region (OAAB17408)
• Molecular Weight: 88061 Da
• Isotype: Ig
• NCBI Gene Id: 6046
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: FSH, NAT, RNF3, FSRG1, RING3, D6S113E, O27.1.1, BRD2-IT1
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: BRD2 is a mitogen-activated kinase which localizes to the nucleus. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3 but sequence comparison suggests that the protein is not involved in the immune response. Homology to the Drosophila gene female sterile homeotic suggests that this human protein may be part of a signal transduction pathway involved in growth control.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: May play a role in spermatogenesis or folliculogenesis (By similarity). Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly
  Cellular Location: Nucleus (Probable). Note=Detected on chromatin and nucleosomes
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This BRD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-200 amino acids from the Central region of human BRD2.
• Intended Use: BRD2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: P25440
• Protein Name: Bromodomain-containing protein 2
• Sample Type Confirmation:

  BRD2 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Protein Accession #: NP_001106653.1
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: BRD2
• Predicted Species Reactivity: Human
• Application: WB, IHC-P
• Predicted Homology Based on Immunogen Sequence: Bovine, Mouse, Rat
• Image 1: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: 293
  
  BRD2-Q185 (Cat.# OAAB17408) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the BRD2 antibody detected the BRD2 protein (arrow).

  BRD2 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Image 3: 293
  
  Western blot analysis of BRD2 (arrow) using rabbit polyclonal BRD2 Antibody (Center) (Cat.# OAAB17408). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRD2 gene (Lane 2) (Origene Technologies).