BRD2 Antibody - middle region (OAAB17408)

Catalog No: OAAB17408

Size:400ul

BRD2-Q185 (Cat.# OAAB17408) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the BRD2 antibody detected the BRD2 protein (arrow).

BRD2 is strongly supported by BioGPS gene expression data to be expressed in HEK293

Western blot analysis of BRD2 (arrow) using rabbit polyclonal BRD2 Antibody (Center) (Cat.# OAAB17408). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRD2 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P
• Additional Information: Function: May play a role in spermatogenesis or folliculogenesis (By similarity). Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly
  Cellular Location: Nucleus (Probable). Note=Detected on chromatin and nucleosomes
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This BRD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-200 amino acids from the Central region of human BRD2.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Bovine, Mouse, Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  BRD2 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Intended Use: BRD2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: BRD2
• Alias Symbols: FSH, NAT, RNF3, FSRG1, RING3, D6S113E, O27.1.1, BRD2-IT1
• NCBI Gene Id: 6046
• Protein Name: Bromodomain-containing protein 2
• Description of Target: BRD2 is a mitogen-activated kinase which localizes to the nucleus. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3 but sequence comparison suggests that the protein is not involved in the immune response. Homology to the Drosophila gene female sterile homeotic suggests that this human protein may be part of a signal transduction pathway involved in growth control.
• Uniprot ID: P25440
• Protein Accession #: NP_001106653.1
• Molecular Weight: 88061 Da