MYLK3 Antibody - N-terminal region (OAAB17351)

• Product Number: OAAB17351
• Name: MYLK3 Antibody - N-terminal region (OAAB17351)
• Molecular Weight: 88393 Da
• Isotype: Ig
• NCBI Gene Id: 91807
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: MLCK, MLCK2, caMLCK
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: MYLK3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility (By similarity)
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This MYLK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from the N-terminal region of human MYLK3.
• Additional Information: Cellular Location: Cytoplasm (By similarity).
  Tissue Location: Restricted to heart.
• Uniprot ID: Q32MK0
• Protein Name: Myosin light chain kinase 3
• Protein Accession #: NP_872299.2
• Gene Symbol: MYLK3
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: Human breast carcinoma
  
  Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MYLK3 antibody (N-term) (Cat.# OAAB17351), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 3: Mouse Heart
  
  Western blot analysis of anti-MYLK3 Antibody (N-term) (Cat. # OAAB17351) in mouse heart tissue lysates (35ug/lane). MYLK3(arrow) was detected using the purified Pab.
• Image 4: A375
  
  Western blot analysis of anti-MYLK3 Antibody (N-term) (Cat. # OAAB17351) in A375 cell line lysates (35ug/lane). MYLK3 (arrow) was detected using the purified Pab.