MYLK3 Antibody - N-terminal region (OAAB17351)

Catalog No: OAAB17351

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Western blot analysis of anti-MYLK3 Antibody (N-term) (Cat. # OAAB17351) in A375 cell line lysates (35ug/lane). MYLK3 (arrow) was detected using the purified Pab.

Western blot analysis of anti-MYLK3 Antibody (N-term) (Cat. # OAAB17351) in mouse heart tissue lysates (35ug/lane). MYLK3(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MYLK3 antibody (N-term) (Cat.# OAAB17351), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: MYLK3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility (By similarity)
  Cellular Location: Cytoplasm (By similarity).
  Tissue Location: Restricted to heart.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This MYLK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from the N-terminal region of human MYLK3.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: MYLK3
• Alias Symbols: MLCK, MLCK2, caMLCK
• NCBI Gene Id: 91807
• Protein Name: Myosin light chain kinase 3
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q32MK0
• Protein Accession #: NP_872299.2
• Molecular Weight: 88393 Da