Product Number |
OAAB17346 |
Product Page |
www.avivasysbio.com/mek2-map2k2-antibody-center-region-oaab17346.html |
Name |
MEK2 Antibody - middle region (OAAB17346) |
Molecular Weight |
44424 Da |
Isotype |
Ig |
NCBI Gene Id |
5605 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Alias Symbols |
CFC4, MEK2, MKK2, MAPKK2, PRKMK2 |
Product Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Description of Target |
Purified Rabbit Polyclonal Antibody (Pab) |
Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for OAAB17346 |
Additional Information |
Precautions: MEK2 (MAP2K2) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Additional Information |
Function: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity) |
Application Info |
WB: 1:1000 IF: 1:10~50 IHC-P: 1:50~100 |
Immunogen |
This MEK2 (MAP2K2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 262-292 amino acids from the Central region of human MEK2 (MAP2K2). |
Uniprot ID |
P36507 |
Protein Name |
Dual specificity mitogen-activated protein kinase kinase 2 |
Sample Type Confirmation |
There is BioGPS gene expression data showing that MAP2K2 is expressed in HEK293 |
Protein Accession # |
NP_109587.1 |
Gene Symbol |
MAP2K2 |
Predicted Species Reactivity |
Human, Mouse |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Human; Mouse |
Image 1 | U251 cell
| Fluorescent image of U251 cell stained with MEK2 (MAP2K2) Antibody (Center)(Cat#OAAB17346/SA110819AW).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEK2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). MEK2 immunoreactivity is localized to Cytoplasm significantly. |
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Image 2 | human cancer tissue
| Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Image 3 | 293 cell
| Western blot analysis of MAP2K2 (arrow) using MAP2K2 Antibody (Center) (Cat.#OAAB17346).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2) (Origene Technologies). |
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Image 4 | mouse liver tissue
| The anti-MAP2K2 Pab (Cat. #OAAB17346) is used in Western blot to detect MAP2K2 in mouse liver tissue lysate. |
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Image 5 | U937 cell
| MAP2K2 Antibody (A278) (Cat. #OAAB17346) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the MAP2K2 antibody detected the MAP2K2 protein (arrow). |
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